Ifih1 (NM_001164477) Mouse Untagged Clone

CAT#: MC220826

Ifih1 (untagged) - Mouse interferon induced with helicase C domain 1 (Ifih1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ifih1
• Synonyms: 9130009C22Rik; Helicard; Hlcd; MDA5; RLR-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220826 representing NM_001164477
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGATTGTCTGTTCTGCAGAGGACAGCTTCAGGAATCTCATCTTATTCTTCAGGCCCAGGCTGAAAA
  TGTACATTCAGGTGGAGCCAGTGCTGGACCACCTCATCTTTCTGTCTGCAGAAACCAAAGAGCAGATTCT
  TAAAAAGATCAACACCTGTGGTAACACCAGCGCGGCAGAACTGCTGCTGAGCACCTTGGAGCAGGGACAA
  TGGCCTCTGGGATGGACGCAGATGTTCGTGGAGGCCCTAGAGCACAGTGGCAATCCCCTAGCCGCGCGCT
  ATGTCAAACCCACACTCACTGATCTGCCCTCTCCTTCCTCTGAGACTGCCCATGACGAGTGTCTCCACTT
  GCTGACCCTCCTCCAGCCCACTTTGGTGGACAAACTTCTGATTAACGATGTCTTGGACACTTGCTTCGAG
  AAGGGACTATTGACAGTCGAAGACAGAAATCGGATTTCTGCTGCAGGAAACAGCGGGAATGAGTCAGGTG
  TAAGAGAGCTGCTGAGAAGGATTGTGCAGAAGGAAAACTGGTTTTCTACCTTCCTGGATGTTCTGCGCCA
  AACTGGAAATGATGCACTATTCCAAGAACTAACAGGTGGAGGCTGCCCAGAAGACAACACAGAATCAGAC
  ACAAGTTTGGCAGAAGGAAGTGTCAGCTGCTTCGATGAAAGTCTTGGACATAACAGCAACATGGGCAGGG
  ATTCAGGCACCATGGGAAGTGATTCAGATGAAAGTGTCATCCAGACAAAAAGAGTATCCCCCGAGCCAGA
  ACTGCAGCTCAGGCCTTACCAAATGGAAGTGGCCCAACCAGCTCTAGATGGGAAGAATATTATTATCTGC
  CTCCCCACGGGGAGTGGGAAAACCAGAGTGGCTGTTTACATCACCAAAGATCACTTAGACAAGAAGAAGC
  AGGCATCTGAATCCGGGAAGGTTATCGTTCTTGTCAATAAGGTAATGTTAGCAGAACAACTTTTCCGAAA
  AGAGTTCAACCCATATTTGAAGAAATGGTATCGAATTATTGGATTAAGTGGCGATACCCAGCTGAAAATA
  TCATTTCCAGAAGTTGTCAAATCTTACGATGTTATTATCAGCACTGCTCAAATCCTTGAAAACTCCCTCT
  TAAATCTGGAGAGTGGAGACGATGACGGTGTGCAGCTGTCAGACTTCTCTCTCATTATCATTGATGAGTG
  CCATCACACCAACAAGGAGGCAGTCTATAACAACATCATGAGACGATATTTGAAGCAGAAGCTGAGAAAC
  AATGACCTCAAGAAACAAAACAAACCAGCCATTCCCCTGCCGCAGATACTAGGACTGACAGCTTCACCTG
  GTGTTGGAGCAGCCAAAAAGCAGTCTGAGGCTGAAAAACATATTTTAAATATATGTGCCAATCTTGATGC
  CTTTACCATTAAAACAGTGAAAGAGAATCTTGGTCAACTCAAACACCAAATAAAGGAACCATGCAAGAAA
  TTTGTGATTGCTGATGACACCAGAGAAAATCCATTTAAAGAGAAACTTCTAGAAATTATGGCAAGCATTC
  AGACTTACTGCCAAAAAAGTCCAATGTCAGATTTTGGAACCCAACATTATGAGCAGTGGGCCATTCAAAT
  GGAGAAAAAAGCTGCTAAAGACGGAAATCGCAAAGATCGCGTCTGTGCAGAACATTTGAGGAAGTACAAC
  GAAGCCCTACAAATCAACGACACGATCCGAATGATTGATGCATATAGCCACCTGGAGACATTCTACACTG
  ATGAGAAAGAAAAGAAGTTCGCAGTCCTCAATGACAGCGACAAGAGTGATGACGAGGCCAGCAGTTGCAA
  TGACCAACTTAAGGGCGATGTAAAGAAATCTTTGAAACTGGACGAAACGGATGAATTTCTCATGAATTTG
  TTCTTTGATAACAAGAAAATGTTGAAAAAACTAGCTGAAAACCCAAAATACGAGAATGAAAAACTCATTA
  AATTAAGAAACACGATACTGGAACAATTCACAAGGTCTGAGGAGTCCTCCCGAGGAATTATTTTTACAAA
  AACACGACAGAGCACCTACGCACTTTCCCAGTGGATCATGGAAAATGCAAAGTTTGCGGAAGTTGGAGTC
  AAAGCGCATCACCTGATTGGCGCGGGGCACAGCAGTGAAGTCAAGCCCATGACTCAGACTGAACAAAAAG
  AAGTCATTAGTAAATTTCGCACTGGCGAAATAAATCTGCTTATCGCTACGACGGTGGCAGAGGAAGGCCT
  GGATATCAAAGAGTGCAATATTGTTATTCGTTATGGCCTTGTCACGAACGAGATAGCCATGGTCCAGGCC
  CGGGGTCGAGCCAGAGCTGATGAAAGCACGTATGTCCTGGTCACCAGCAGTGGCTCAGGAGTTACCGAAC
  GGGAGATTGTTAATGATTTCCGAGAGAAGATGATGTATAAAGCTATTAACCGTGTTCAAAACATGAAACC
  AGAGGAGTATGCACATAAGATTTTGGAATTGCAGGTGCAAAGTATCCTGGAAAAGAAAATGAAAGTCAAA
  AGAAGCATTGCAAAGCAATACAACGACAATCCATCGTTAATAACACTTCTCTGCAAAAATTGTAGCATGC
  TGGTCTGCTCGGGAGAAAACATCCATGTCATTGAGAAGATGCATCATGTCAATATGACACCAGAATTCAA
  GGGACTCTACATTGTAAGAGAAAACAAAGCACTGCAAAAGAAATTTGCTGATTATCAGACCAATGGAGAG
  ATTATCTGCAAGTGTGGCCAGGCTTGGGGAACAATGATGGTGCACAAAGGTTTAGATTTGCCTTGTCTTA
  AAATAAGGAATTTTGTAGTCAATTTCAAAAATAACTCACCGAAGAAACAGTACAAGAAGTGGGTGGAATT
  GCCTATCAGATTTCCTGATCTTGACTACTCAGAATACTGCTTGTATAGTGATGAAGATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164477
• Insert Size: 2931 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164477.1, NP_001157949.1
• RefSeq Size: 5372 bp
• RefSeq ORF: 2931 bp
• Locus ID: 71586
• UniProt ID: Q8R5F7
• Cytogenetics: 2 C1.3
• Gene Summary: Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and theiler's murine encephalomyelitis virus (TMEV). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.