Flvcr1 (NM_001081259) Mouse Untagged Clone

CAT#: MC219012

Mfsd7b (untagged) - Mouse major facilitator superfamily domain containing 7B (Mfsd7b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Flvcr1
• Synonyms: 9630055N22Rik; FLVCR; Flvcr1; Mfsd7b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219012 representing NM_001081259
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGGCCCGATGACGAGGTGGGGCCGGCCGTGGCGCCCGGGCACCCGCTGGGGAAGGGATACCTTC
  CAGTGCCCAAGGGCGCCCCGGACGGGGAGGCGCGCTTGGTACCGCAAAACGGGCCTGAGGCGCTGAACGG
  AGGCCCCGGGCTCGGCCCGCTGATCGCGGGAGCCCAGGGCGGGCCGCAAGCCCTGATAGCGGCGGAGGAG
  GAGACCCAGGCCCGGCTGTTGCCCGCGGGCGACGGGGAGGACGTCCCATGCCCCGCGTGTCCCCCGCGCA
  CAGCGCTGTCGCCGAGGCGCTTCGTGGTGCTGCTCATCTTCAGCCTTTACTCGCTGGTGAACGCCTTCCA
  GTGGATCCAGTACAGCAGCATCAGCAACGTGTTCGAGGACTTCTACGAGGTGTCGCCGCTGCACATCAAC
  TGGCTGTCCATGGTGTACATGGTGGCCTACGTGCCCCTCATCTTCCCGGCCACCTGGCTGCTGGACACGC
  GAGGCCTGCGGCTCACGGCGCTGCTGGGCTCCGGCCTCAACTGCCTGGGAGCCTGGGTCAAGTGCGGCAG
  CGTGCAGCGGCACCTCTTCTGGGTCACCATGCTGGGGCAGATCCTGTGCTCCGTGGCCCAGGTCTTCATC
  CTGGGCTTGCCCTCCCCCGTCGCCTCGGTATGGTTTGGACCCAAGGAGGTGTCGACGGCTTGTGCCACAG
  CAGTGCTGGGCAATCAGCTTGGAACTGCAGTTGGTTTTTTGTTGCCACCTGTTTTAGTGCCTGCCTTGGG
  CACACAGAATAGCACAGGCCTCCTGGCTCACACACAGAATAACACAGACCTCCTGGCTCATAACATCAAC
  ACCATGTTTTACGGAACAGCATTTATCTCCACATTTTTATTTTTTTTAACAATAATTGCATTCAAGGAGA
  AACCTCCGTTGCCTCCAAGTCAGGCTCAGGCAGTTCTTCGAGACAGCCCCCCTGAAGAGTACTCCTACAA
  GAGTTCTATCTGGAACCTGTGCAGAAACATCCCCTTTGTTCTCCTGCTGGTCAGTTATGGTATCATGACT
  GGAGCATTTTATTCAATCTCAACATTATTGAATCAAATAATATTGACATATTATGTGGGAGAAGAGGTGA
  ATGCTGGGAGGATCGGGCTGACACTGGTGGTAGCTGGAATGGTGGGCTCTATTCTCTGTGGCTTATGGCT
  GGACTATACCAAAACCTACAAGCAGACAACTCTGATAGTGTACGTTCTGTCTTTCATTGGAATGCTCATA
  TTCACATTCACACTGAACCTCGGGTACATCATCGTGGTGTTTTTTACTGGTGGGATTCTTGGCTTCTTCA
  TGACTGGTTACCTCCCGCTGGGCTTTGAGTTTGCTGTGGAGATCACCTACCCTGAGTCTGAGGGCATGTC
  CTCAGGGCTCCTCAACACTGCTGCACAGATACTTGGAATTTTCTTCACATTGGCTCAAGGAAAGATCACA
  ACAGACTATAATAGTCCAGAGGCAGGAAACATTTTCCTTTGTGCCTGGATGTTTGTAGGCATAATTTTAA
  CAGCGTTGATCAAGTCTGATCTGAGAAGGCACAATATAAACACCGGGCTTACAAACATTGACGTTAAAGC
  TGTACCAGTTGATAGTCGGGTAGATCCAAAACCCAAAGTGATGGTGTCTATACAGTCGGAATCTTCACTC
  TGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081259
• Insert Size: 1683 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081259.1, NP_001074728.1
• RefSeq Size: 4087 bp
• RefSeq ORF: 1683 bp
• Locus ID: 226844
• UniProt ID: B2RXV4
• Cytogenetics: 1 H6
• Gene Summary: Isoform 1: Heme transporter that exports cytoplasmic heme. It can also export coproporphyrin and protoporphyrin IX, which are both intermediate products in the heme biosynthetic pathway. Does not export bilirubin. Heme export depends on the presence of HPX and is required to maintain intracellular free heme balance, protecting cells from heme toxicity. Heme export provides protection from heme or ferrous iron toxicities in liver, brain, sensory neurons and during erythtopoiesis, a process in which heme synthesis intensifies. Causes susceptibility to FeLV-C in vitro.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).