Ackr4 (NM_145700) Mouse Untagged Clone

CAT#: MC213257

Ackr4 (untagged) - Mouse chemokine (C-C motif) receptor-like 1 (Ccrl1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ackr4
• Synonyms: A630091E18Rik; CCBP2; CCR11; Ccrl1; CCX-CKR; CCX-CKR1; Cmkbrl1; PPR1; VSHK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC213257 representing NM_145700
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGGAGCTGAACCAGTCAGCGGAATACTATTATGAGGAGAATGAGATGAACTACACTCACGACT
  ACAGCCAGTACGAAGTGATCTGCATAAAAGAAGAGGTCAGGCAGTTCGCAAAAGTCTTCCTGCCTGCCTT
  CTTCACAGTGGCCTTTGTCACTGGGCTCGCAGGGAACTCCGTAGTTGTGGCGATTTACGCCTATTACAAG
  AAGCAGAGGACCAAGACCGATGTGTACATCCTGAACCTGGCTGTAGCAGACTTGTTACTTCTGATCACGC
  TGCCTTTCTGGGCAGTTAATGCAGTTCACGGATGGATTCTAGGCAAAATGATGTGCAAAGTAACGTCAGC
  CCTGTACACGGTAAACTTTGTCTCTGGGATGCAGTTCCTGGCTTGTATCAGCATTGACAGATATTGGGCA
  ATTACCAAAGCCCCCAGCCAATCAGGAGCGGGGAGACCCTGTTGGATCATCTGTTGCTGTGTGTGGATGG
  CCGCCATCTTGCTGAGCATACCCCAGCTGGTTTTTTACACAGTGAATCAGAATGCTAGGTGCACTCCCAT
  CTTTCCCCACCACCTAGGAACATCCCTGAAAGCATCCATTCAGATGCTGGAAATCGGCATCGGCTTTGTG
  GTCCCGTTTCTCATCATGGGCGTGTGCTATGCCAGTACCGCCAGGGCGCTCATCAAGATGCCCAACATTA
  AAAAGTCTCGCCCCCTCAGGGTTCTGCTCGCGGTGGTGGTGGTTTTCATTGTCACCCAGCTGCCCTATAA
  CGTTGTTAAGTTCTGCCAAGCCATAGATGCCATCTACCTGCTGATCACCAGCTGCGATATGAGCAAACGC
  ATGGATGTCGCCATCCAAGTCACAGAGAGCATCGCGCTCTTCCACAGCTGCCTCAACCCCATCCTGTATG
  TCTTCATGGGGGCCTCCTTCAAAAACTATATCATGAAAGTGGCCAAGAAATATGGATCCTGGAGAAGACA
  GAGACAGAACGTGGAAGAAATTCCTTTTGATTCTGAGGGTCCTACAGAGCCAACCAGTTCTTTTACCATT
  TAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145700
• Insert Size: 1053 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145700.2, NP_663746.2
• RefSeq Size: 2109 bp
• RefSeq ORF: 1053 bp
• Locus ID: 252837
• UniProt ID: Q924I3
• Cytogenetics: 9 F1
• Gene Summary: Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CCL2, CCL8, CCL13, CCL19, CCL21 and CCL25. Chemokine-binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization. Plays an important role in controlling the migration of immune and cancer cells that express chemokine receptors CCR7 and CCR9, by reducing the availability of CCL19, CCL21, and CCL25 through internalization. Negatively regulates CXCR3-induced chemotaxis. Regulates T-cell development in the thymus and inhibits spontaneous autoimmunity.[UniProtKB/Swiss-Prot Function]