Gpt (NM_182805) Mouse Untagged Clone

CAT#: MC205377

Gpt (untagged) - Mouse glutamic pyruvic transaminase, soluble (Gpt), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gpt
• Synonyms: ALT; ALT1; Gpt-1; Gpt1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC022625
  CCCACGCGTCCGGTGCCTTACCTCACGCAGACTCCCCATTCCCAGCCCTGCTCCCCAACTGGACCCTTCT TCTTGAGCCAGCATTCTTGCCTGCTTTGAGCAGCCATGGCCTCACAAAGGAATGACCGGATCCAGGCTTC AAGGAATGGACTGAAGGGGAAGGTGCTAACTCTGGATACCATGAACCCATGTGTGCGGAGGGTGGAGTAT GCAGTCCGAGGCCCCATCGTGCAACGTGCCTTGGAGCTGGAGCAGGAGCTGCGCCAGGGTGTGAAGAAGC CTTTTACTGAGGTTATCCGTGCCAATATTGGGGATGCACAAGCCATGGGGCAGAGACCCATCACCTTCTT CCGCCAGGTCCTGGCCCTCTGTGTCTACCCCAATCTTCTGAGCAGTCCGGACTTCCCAGAGGATGCCAAG AGAAGGGCAGAACGCATCTTGCAGGCATGCGGGGGCCACAGCCTGGGTGCCTATAGCATTAGCTCTGGAA TCCAGCCGATTCGGGAGGATGTGGCGCAATATATTGAGAGGAGAGACGGAGGCATCCCTGCAGACCCGAA CAACATATTTCTGTCCACAGGGGCCAGCGATGCCATCGTGACCATGCTCAAGCTGCTGGTAGCCGGCGAG GGCCGTGCGCGAACCGGTGTACTCATTCCCATTCCTCAGTACCCACTGTACTCAGCTGCGCTGGCTGAGC TGGACGCCGTGCAAGTGGACTACTACCTGGACGAAGAGCGCGCCTGGGCTCTTGACATCGCTGAGCTGCG GCGCGCTCTGTGCCAGGCACGTGACCGCTGCTGCCCTCGAGTACTATGCGTCATCAACCCCGGCAACCCC ACGGGGCAGGTGCAGACCCGTGAATGCATCGAGGCCGTAATCCGCTTTGCTTTCGAAGAGGGACTCTTCC TGATGGCTGATGAGGTATACCAAGACAATGTATATGCCGAGGGCTCTCAGTTCCATTCATTCAAGAAGGT GCTCACGGAGATGGGGCCACCATATGCCACGCAGCAGGAGCTCGCGTCTTTCCACTCAGTCTCTAAGGGC TACATGGGCGAGTGCGGGTTTCGTGGTGGCTATGTGGAAGTGGTAAACATGGATGCCGAGGTGCAGAAAC AGATGGCGAAACTGATGAGCGTGCGGTTGTGTCCACCAGTGCCGGGCCAGGCTTTGATGGGCATGGTGGT CAGTCCGCCAACCCCCTCGGAGCCGTCCTTCAAGCAGTTTCAAGCAGAGAGGCAGGAGGTGCTGGCTGAA CTGGCAGCCAAGGCTAAACTCACGGAGCAGGTCTTCAACGAGGCCCCCGGGATCCGCTGCAACCCGGTGC AGGGCGCTATGTATTCCTTCCCTCAAATTCAGCTGCCTTTGAAAGCAGTGCAGCGTGCGCAGGACCTGGG CCTGGCCCCTGACATGTTCTTCTGTCTGTGCCTCCTGGAAGAGACTGGCATCTGCGTTGTGCCTGGGAGT GGCTTTGGGCAGCAGGAGGGCACCTATCATTTCCGGATGACCATTCTGCCCCCCATGGAGAAACTGCGGG TGCTGCTGGAGAAACTGAGGCACTTCCATGCTAAATTCACTCATGAGTACTCCTGAAGCCACTGCTAGGG CCACACTGGATGCTCTCTGACTCAACAAACTGAGGGTCCTTGGGAGCCCTCACTATTTCTGATTTTGCAT AGGGTCTGGGTACTTGTCCCTGCAGGTCCCTAATAAAGCTGGGTGTAGGCCTGATTTGAGGTGGCTGCCT GGGTGACCCCAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_182805
• Insert Size: 1491 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC022625, AAH22625
• RefSeq Size: 1784 bp
• RefSeq ORF: 1491 bp
• Locus ID: 76282
• UniProt ID: Q8QZR5
• Cytogenetics: 15 36.28 cM
• Gene Summary: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).[UniProtKB/Swiss-Prot Function]