Tmprss2 (NM_015775) Mouse Untagged Clone

CAT#: MC204660

Tmprss2 (untagged) - Mouse transmembrane protease, serine 2 (Tmprss2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tmprss2
• Synonyms: D16Ertd61e
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC054348
  GCCTTTCCTGGCCGTTCCCTCCTTCTGGCCGAGGTGCCTGCGTTTAGGGGTGTCACCCTGGCTCCCGGGA CGCCGCCTCCGGAGATTTAAGCGAGAACTGGAGTAGGTCGTGTACTTGGAGCGGACGAGGAAGCCAAGAG CTCGGACAGAGGCGGAGAGGGGCGGGAAGCGCAACAGGTCACCTGGAGGAAGCCCCATACTGACCTCCTC ATGCTGCTGACACAGGCAGGATGGCATTGAACTCAGGGTCACCTCCAGGAATCGGACCTTGCTATGAGAA CCACGGGTATCAGTCTGAGCACATCTGTCCTCCGAGACCACCAGTGGCTCCCAATGGCTACAACTTGTAT CCAGCCCAGTACTACCCATCTCCAGTGCCTCAGTATGCTCCGAGGATTACAACGCAAGCCTCAACATCTG TCATCCACACACATCCCAAGTCCTCAGGAGCACTGTGCACCTCAAAGTCTAAGAAATCGCTGTGTTTAGC CCTCGCCCTGGGCACTGTCCTCACGGGAGCTGCTGTGGCTGCTGTCTTGCTTTGGAGGTTCTGGGACAGC AACTGTTCTACGTCTGAGATGGAGTGTGGGTCTTCAGGCACATGCATCAGCTCTTCTCTCTGGTGTGACG GGGTAGCACATTGTCCCAACGGAGAAGATGAGAACCGTTGTGTTCGTCTCTACGGACAAAGCTTCATCCT CCAGGTTTACTCATCTCAGAGGAAAGCCTGGTATCCCGTGTGCCAGGATGATTGGAGTGAGAGCTACGGG AGAGCAGCATGTAAAGACATGGGATACAAGAACAATTTTTATTCTAGCCAAGGGATACCAGACCAGAGCG GGGCAACGAGCTTTATGAAGCTGAATGTGAGCTCAGGCAACGTTGACCTCTATAAAAAACTCTACCACAG TGACTCATGTTCATCCCGCATGGTGGTTTCTTTGCGCTGTATAGAATGCGGGGTTCGCTCAGTGAAACGC CAGAGCAGGATTGTGGGTGGATTGAATGCCTCACCAGGAGACTGGCCCTGGCAGGTCAGCCTGCACGTCC AAGGCGTCCACGTCTGCGGAGGCTCCATCATCACCCCCGAGTGGATTGTGACGGCCGCCCACTGTGTGGA AGAACCCCTCAGCAGCCCGAGGTACTGGACGGCATTTGCGGGAATTCTGAGACAGTCTCTCATGTTCTAT GGAAGTAGACACCAGGTAGAAAAAGTAATTTCCCATCCAAATTACGACTCTAAGACCAAGAATAACGACA TTGCTCTCATGAAGCTGCAGACACCTTTGGCTTTTAATGATCTAGTGAAGCCAGTGTGTCTGCCGAACCC AGGCATGATGCTAGACCTAGACCAGGAATGCTGGATTTCGGGGTGGGGGGCCACCTATGAGAAAGGGAAG ACCTCGGACGTGTTGAATGCTGCCATGGTACCCTTGATCGAGCCCTCCAAATGTAATAGTAAATACATAT ACAACAACCTAATCACACCAGCCATGATCTGTGCCGGCTTCCTCCAGGGGTCTGTCGACTCTTGCCAGGG AGACAGTGGAGGGCCGCTGGTTACTTTGAAGAATGGGATCTGGTGGCTGATTGGGGACACGAGCTGGGGC TCGGGCTGTGCCAAGGCACTCAGACCTGGAGTATACGGGAACGTGACGGTATTTACAGATTGGATCTACC AGCAAATGAGGGCGAACAGCTAATCCACGTGGCTTTGTCCCAGACTTCCTTTGTCTTCAACAACCTTCTG CAAGAAAACCAAGGGCCTGAATTTTAACTTCCTGTGCACAATGTACCTTTTGAGATGATTCGAAGGGCCT TTCACTTTTATTAAACAGTGACTTGTTTGACTGTGCTCCCTGGTCCTGTGAGGGCTTCAGTGCCCCACCC CTGGGCCACTTCTGCAGCTCCCACCAGAATGGATGACCAGATTCTGTTGGGTTTGGGCACATAGGGCCAA AGGCAGAGGAGGGTGGCACTCTCATGTTGGAACTTCTTTTGGGCTCATGCTCAGGCCTTTTTTGGATCAC TAAGGACTATGACCTCTGAGTAACCTGATGACCTGAGAAAGAGTAAGGAGGCCAGGCAGGGCCTTGGGCC CAGGAACAGGTACCTTGAGAGTGAGAGCTACCCATTGCCTGTGGCCTAAATCTGCTGTGCAGGTTGGGCT GGTCATACTGTCATGATTTCATTAACAGCCTGGGTGAACATGGCTGGGAGTAAAGGGCTTGCTCTCCTGC ATGTTGACATGACGGCCCTTTCCAAGGGTGATGGAGGCTTTCCCAAGCTAAGGGCCTAGGCAGATCTCTC AGAGCAAGAAGCTAATGCCGGCATGTCCCTTGGGTGAGCTCTACATGGTGTTATTCAGTCTGGTTCTTGG CTCCCCACTACTGTTTCTCTCAGCCTCTCAGAGCCTGAAACTTACCTCTTAGCTTTGGCTACAGGCATGG CCTAGTACCTGATGGAGCCTGTATAGCTCAGCTAATCAAATGGAGGCTCAGGTCCATCAGAATCAGGGAC TTGTGATTTCAGTCACCTTGCTTCTGGGTTGTGTTTCTTCTCTTACTACCTCACTGCACCTGGACACTAG AGTGGATGAATGTCTGGAGTTCACCTGCATTTGGACTGTGTGATTGTGCCTCAGACACTAGACCTCTTCC AGATGGTTAGGTTGTTCTGTAGACTGGCAATGAGATTAGAAGTTCCTAGCTTCAGATAAAGATGAAAGAG AGGAGATCATTGTCTTCTGTCTTCTTCTGGCCCTGGGTTTATACCAGGAAAGCCATGCCAGAATTACCAA ATATGAAGTATGAATGTCTTACCCACGGTGAGGCTCTGCCTCCTTCTCTCTGCCTGGTTCTTCAGAAGGC AGTGAATGGGTCATAACTGGGACTCCATCTTTGCTGGGGAAAGTCTCCCACCTAGGGAATGGTTACCACT CCATGTAAAGAAAACTCCCTCATGCGTCCTCTGGGACCTTCTTAGATGCTGTAAGGTACCTACATACAGA CTAAATGTGCAAGCACCTTGAAGTGTGAGAACCTGTCCCCTCCTTAGCTCTCCTTGTCTTTGCTGTTGGT TGGTTATTTCCTGCTTTGTGTCTGTTCTGAGCTGTGAGATTCCACTGTGAAATATATGAATAAAGTATAT AATTCTTTTAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_015775
• Insert Size: 1473 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC054348, AAH54348
• RefSeq Size: 3175 bp
• RefSeq ORF: 1473 bp
• Locus ID: 50528
• UniProt ID: Q9JIQ8
• Cytogenetics: 16 57.53 cM
• Gene Summary: Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions. The spike proteins are synthesized and maintained in precursor intermediate folding states and proteolysis permits the refolding and energy release required to create stable virus-cell linkages and membrane coalescence. Facilitates human SARS coronavirus (SARS-CoV) infection via two independent mechanisms, proteolytic cleavage of ACE2, which might promote viral uptake, and cleavage of coronavirus spike glycoprotein which activates the glycoprotein for cathepsin L-independent host cell entry. Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9) and is involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.[UniProtKB/Swiss-Prot Function]