Orc4 (NM_011958) Mouse Untagged Clone

CAT#: MC200807

Orc4 (untagged) - Mouse origin recognition complex, subunit 4 (Orc4), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Orc4
• Synonyms: mMmORC4; Orc4l; Orc4P
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC015072 sequence for NM_011958
  CCACGCGTCCGCCACGCGTCCGGCAGTAGGAGCAGCGGGACTGAGAAGGTGGTGAACATCCTTCGGTCCT CACCGTCGGCGGCGGCCGGCTTTGCCCGATTTAGGCAGGCTTGACTGGGAGAGGATTTGAATTAATCGAG ATGAGCAGTCGTAAAACCAAGAGTAATGCACATGCAGAGTGTCTTTCACAGGTACAAAGAATTTTACGTG AAAGATTTTGTCATCATAGTCCACATAGCAACCTCTTTGGAGTGCAAGTACAATACAAGCATTTGATTGA GCTACTCAAAAGAACTGCTATCTATGGAGAAAGCAATTCTGTACTCATTGTTGGACCACGAGGATCAGGA AAGACCACATTATTAAATCATGCTTTGAAAGAACTCATGGAAATAGAAGTGAGTGAAAATGTGATACAAG TTCACTTAAATGGACTCCTGCAGACAAATGAAAAAATTGCTCTGAAAGAGATCACAAGACAATTAAATCT AGACAATGTAGTTGAAGATAAAGTTTTTGGAAGCTTTGCTGAAAACCTTTCATTTCTTCTGGAAGCTTTG CAAAAAGGTGACCGGACTAGCAGTTGCCCAGTGATCTTCATACTGGATGAATTTGATATTTTTGCTCATC AGAAAAATCAAACACTCCTTTATAATCTTTTTGACATTTCTCAATCTGCACAGACACCAGTAGCAGTTAT TGGACTTACATGTAGATTGGATATTTTGGAACTCTTAGAAAAGAGAGTGAAGTCACGATTTTCTCACCGG CAGATACATTTAATGAATTCATTTGATTTTCCACAATATTTGAAAATATTTAAAGAACAGTTATCTCTAC CTGCAGAATTCCCAGATAAGGCTTTTGCTGAGAGATGGAATGAGAATGTTCACTGTCTCTCTGAAGATTC AACTGTGCTTGAAGTCCTACAGAAACATTTCAGTGTCAACAAAAACTTGCAGTCATTACACATGCTATTG ATGCTTGCTTTAAATCGAGTAACCGTATCACACCCATTTATGACTTCAGCAGATCTGATGGAGGCACAGC ATATGTGTAGCTTGGATTCTAAGGCGAATATTGTACATGGTCTGTCAGTCTTGGAAATCTGTCTTATAAT AGCAATGAAACATTTAAATGACATATATGAAGAGGAGCCCTTTAATTTTCAAATGGTGTATAATGAATTT CAGAAATTCATTCAAAGAAAGGCCCATTCTGTTTATAACTTTGAGAAACCTGTGGTCATGAAGGCATTTG AGCACTTACAACAGTTGGAATTAATAAAACCCGTGGAAAGAACTTCAGTAAATTCTCAGAGAGAATACCA GCTAGTGAAACTACTTTTGGATAATACTCAAATTATGAATGCTCTACAGAAATACTCCAACTGCCCTACA GATGTTAGGCAGTGGGCAACATCCTCACTAAGCTGGCTGTGAAGATTTTAAGTACATTTCTGTGAAGACC TTAAAGTACTGTTCTTTATTTAATAAGATACGCTACTACATTGCTTTGTTTATAAATATGTTTATGGGAA ATTTGCACTTAGTATGTTTTGAATAATGACCACGCATGGACACAGTTTCATTAATTTAAATTGCACCACA CAAGTAGCAGTTCATAAGAATAAAATGTGCTTATTTTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011958
• Insert Size: 1302 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC015072, AAH15072
• RefSeq Size: 1662 bp
• RefSeq ORF: 1302 bp
• Locus ID: 26428
• UniProt ID: O88708
• Cytogenetics: 2 C1.1
• Gene Summary: Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 (By similarity). Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1 and 4 both encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.