Mouse Pdha1 (NM_008810) AAV Particle

CAT#: MR206079A1V

AAV ORF Particles, serotype AAV-2, Pdha1 (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase E1 alpha 1 (Pdha1), nuclear gene encoding mitochondrial protein, 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Pdha1
• Synonyms: Pdha-1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206079 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAGATGCTTGCCGCTGTATCCCGCGTGTTGGCAGGCTCTGCGCAGAAGCCGGCAAGCCGAGTGC
  TGGTTGCTTCCCGTAATTTTGCAAATGATGCTACATTTGAGATTAAGAAATGTGACCTTCATCGGCTAGA
  AGAGGGCCCCCCAGTCACCACAGTGCTCACCAGAGAGGATGGGCTCAAGTACTACAGGATGATGCAGACT
  GTGCGCCGGATGGAGCTAAAGGCGGATCAGCTGTATAAGCAGAAAATCATTCGTGGTTTCTGTCACTTGT
  GTGATGGTCAGGAAGCCTGCTGCGTGGGCCTGGAGGCTGGCATAAACCCTACGGACCACCTCATCACTGC
  CTATCGAGCACATGGCTTCACCTTCACTCGGGGCCTGCCTGTGCGAGCAATTCTTGCAGAGCTAACAGGA
  CGAAGAGGAGGTTGTGCTAAAGGGAAAGGCGGGTCAATGCACATGTACGCCAAGAACTTCTATGGAGGCA
  ACGGCATCGTTGGAGCTCAGGTGCCCCTGGGAGCAGGAATTGCCCTGGCCTGCAAGTACAATGGAAAAGA
  TGAGGTCTGTTTGACATTATACGGCGATGGTGCTGCTAATCAGGGTCAGATCTTTGAAGCTTACAATATG
  GCAGCACTGTGGAAATTACCTTGCATTTTTATCTGTGAGAACAACCGCTATGGCATGGGGACGTCTGTTG
  AGAGAGCAGCAGCCAGCACGGACTACTACAAAAGAGGAGATTTTATTCCTGGACTCAGGGTAGATGGAAT
  GGATATCTTGTGCGTCCGAGAGGCAACAAAGTTTGCGGCTGCCTATTGCAGGTCTGGTAAGGGGCCCATC
  CTGATGGAGCTCCAGACTTACCGCTACCATGGACACAGCATGAGTGACCCTGGAGTAAGCTACCGCACTC
  GAGAAGAAATCCAGGAAGTAAGAAGTAAGAGTGACCCTATTATGCTTCTCAAGGATAGAATGGTGAACAG
  CAATCTTGCAAGTGTTGAAGAATTAAAGGAGATTGATGTGGAAGTGAGGAAAGAAATCGAGGATGCTGCC
  CAGTTTGCCACGGCTGATCCTGAGCCCCCGTTGGAGGAACTAGGCTATCACATCTACAGCAGTGATCCTC
  CCTTTGAAGTGCGTGGTGCCAACCAGTGGATCAAGTTTAAGTCAGTCAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206079 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRKMLAAVSRVLAGSAQKPASRVLVASRNFANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQT
  VRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLPVRAILAELTG
  RRGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNM
  AALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATKFAAAYCRSGKGPI
  LMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAA
  QFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_008810
• ORF Size: 1173 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_008810.2
• RefSeq Size: 2778 bp
• RefSeq ORF: 1173 bp
• Locus ID: 18597
• UniProt ID: P35486
• Cytogenetics: X 73.95 cM
• MW: 43.2 kDa