ADAM12 (NM_003474) Human 3' UTR Clone

CAT#: SC220027

3' UTR clone of ADAM metallopeptidase domain 12 (ADAM12) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ADAM12
• Synonyms: ADAM12-OT1; CAR10; MCMP; MCMPMltna; MLTN; MLTNA
• ACCN: NM_003474
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC220027 3’UTR clone of NM_003474
  The sequence shown below is from the reference sequence of NM_003474. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AGATCCACCCACACCGCCTATATTAAGTGAGAAGCCGACACCTTTTTTCAACAGTGAAGACAGAAGTTT
  GCACTATCTTTCAGCTCCAGTTGGAGTTTTTTGTACCAACTTTTAGGATTTTTTTTAATGTTTAAAACA
  TCATTACTATAAGAACTTTGAGCTACTGCCGTCAGTGCTGTGCTGTGCTATGGTGCTCTGTCTACTTGC
  TCAGGTACTTGTAAATTATTAATTTATGCAGAATGTTGATTACAGTGCAGTGCGCTGTAGTAGGCATTT
  TTACCATCACTGAGTTTTCCATGGCAGGAAGGCTTGTTGTGCTTTTAGTATTTTAGTGAACTTGAAATA
  TCCTGCTTGATGGGATTCTGGACAGGATGTGTTTGCTTTCTGATCAAGGCCTTATTGGAAAGCAGTCCC
  CCAACTACCCCCAGCTGTGCTTATGGTACCAGATGCAGCTCAAGAGATCCCAAGTAGAATCTCAGTTGA
  TTTTCTGGATTCCCCATCTCAGGCCAGAGCCAAGGGGCTTCAGGTCCAGGCTGTGTTTGGCTTTCAGGG
  AGGCCCTGTGCCCCTTGACAACTGGCAGGCAGGCTCCCAGGGACACCTGGGAGAAATCTGGCTTCTGGC
  CAGGAAGCTTTGGTGAGAACCTGGGTTGCAGACAGGAATCTTAAGGTGTAGCCACACCAGGATAGAGAC
  TGGAACACTAGACAAGCCAGAACTTGACCCTGAGCTGACCAGCCGTGAGCATGTTTGGAAGGGGTCTGT
  AGTGTCACTCAAGGCGGTGCTTGATAGAAATGCCAAGCACTTCTTTTTCTCGCTGTCCTTTCTAGAGCA
  CTGCCACCAGTAGGTTATTTAGCTTGGGAAAGGTGGTGTTTCTGTAAGAAACCTACTGCCCAGGCACTG
  CAAACCGCCACCTCCCTATACTGCTTGGAGCTGAGCAAATCACCACAAACTGTAATACAATGATCCTGT
  ATTCAGACAGATGAGGCTTTCCATGGGACCACAACTATTTTCAGATGTGAACCATTAACCAGATCTAGT
  CAATCAAGTCTGTTTACTGCAAGGTTCAACTTATTAACAATTAGGCAGACTCTTTATGCTTGCAAAAAC
  TACAACCAATGGAATGTGATGTTCATGGGTATAGTTCATGTCTGCTATCATTATTCGTAGATATTGGAC
  AAAGAACCTTCTCTATGGGGCATCCTCTTTTTCCAACTTGGCTGCAGGAATCTTTAAAAGATGCTTTTA
  ACAGAGTCTGAACCTATTTCTTAAACACTTGCAACCTACCTGTTGAGCATCACAGAATGTGATAAGGAA
  ATCAACTTGCTTATCAACTTCCTAAATATTATGAGATGCTGGCTTGGGCAGCATCCCCTTGAACTCTTC
  ACTCTTCAAATGCCTGACTAGGGAGCCATGTTTCACAAGGTCTTTAAAGTGACTAATGGCATGAGAAAT
  ACAAAAATACTCAGATAAGGTAAAATGCCATGATGCCTCTGTCTTCTGGACTGGTTTTCACATTAGAAG
  ACAATTGACAACAGTTACATAATTCACTCTGAGTGTTTTATGAGAAAGCCTTCTTTTGGGGGTCAACAG
  TTTTCCTATGCTTTGAAACAGAAAAATATGTACCAAGAATCTTGGTTTGCCTTCCAGAAAACAAAACTG
  CATTTCACTTTCCCGGTGTTCCCCACTGTATCTAGGCAACATAGTATTCATGACTATGGATAAACTAAA
  CACGTGACACAAACACACACAAAAGGGAACCCAGCTCTAATACATTCCAACTCGTATAGCATGCATCTG
  TTTATTCTATAGTTATTAAGTTCTTTAAAATGTAAAGCCATGCTGGAAAATAATACTGCTGAGATACAT
  ACAGAATTACTGTAACTGATTACACTTGGTAATTGTACTAAAGCCAAACATATATATACTATTAAAAAG
  GTTTACAGAATTTTATGGTGCATTACGTGGGCATTGTCTTTTTAGATGCCCAAATCCTTAGATCTGGC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003474.6
• Summary: This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014]
• Locus ID: 8038
• MW: 75.4