AREB6 (ZEB1) (NM_001128128) Human 3' UTR Clone

CAT#: SC219122

3' UTR clone of zinc finger E-box binding homeobox 1 (ZEB1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: AREB6
• Synonyms: AREB6; BZP; DELTAEF1; FECD6; NIL2A; PPCD3; TCF8; ZFHEP; ZFHX1A
• ACCN: NM_001128128
• Insert Size: 2593 bp
• Sequence Data:
  Insert Sequence

  >SC219122 3' UTR clone of NM_001128128
  The sequence shown below is from the reference sequence of NM_001128128. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CGAGAGTAGTGAGCAAGTGTCTGAAGAAAAGACAAATGAAGCCTAATCGTTTTTCTAGAAGGAAAATAAA
  TTCTAATTGATAATGAATTTCGTTCAATATTATCCTTGCTTTTCATGGAAACACAGTAACCTGTATGCTG
  TGATTCCTGTTCACTACTGTGTAAAGTAAAAACTAAAAAAATACAAAATACAAAACACACACACACACAC
  ACACACACACACACACACACACACAAAATAAATCCGGGTGTGCCTGAACCTCAGACCTAGTAATTTTTCA
  TGCAGTTTTCAAAGTTAGGAACAAGTTTGTAACATGCAGCAGATTAGAAAACCTTAATGACTCAGAGAGC
  AACAATACAAGAGGTTAAAGGAAGCTGATTAATTAGATATGCATCTGGCATTGTTTTATCTTATCAGTAT
  TATCACTCTTATGTTGGTTTATTCTTAAGCTGTACAATTGGGAGAAATTTTATAATTTTTTATTGGTAAA
  CATATGCTAAATCCGCTTCAGTATTTTATTATGTTTTTTAAAATGTGAGAACTTCTGCACTACAAAATTC
  CCTTCACAGAGAAGTATAATGTAGTTCCAACCCGTGCTAACTACCTTTTATAAATTCAGTCTAGAAGGTA
  GTAATTTCTAATATTTAGATGTCTTAGTAGAGCGTATTATCATTTAAAGTGTATTGTTAGCCTTAAGAAA
  GCAGCTGATAGAAGAACTGAAGTTTCTTACTCACGTGGTTTAAAATGGAGTTCAAAAGATTGCCATTGAG
  TTCTGATTGCAGGGACTAACAATGTTAATCTGATAAGGACAGCAAAATCATCAGAATCAGTGTTTGTGAT
  TGTGTTTGAATATGTGGTAACATATGAAGGATATGACATGAAGCTTTGTATCTCCTTTGGCCTTAAGCAA
  GACCTGTGTGCTGTAAGTGCCATTTCTCAGTATTTTCAAGGCTCTAACCCGCCTTCATCCAATGTGTGGC
  CTACAATAACTAGCATTTGTTGATTTGTCTCTTGTATCAAAATTCCCAAATAAAACTTAAAACCACTGAC
  TCTGTCAGAGAAACTGAAACACTGGGACATTTCATCCTTCAATTCCTCGGTATTGATTTTATGTTGATTG
  ATTTTCAGAATTTCTCTACAGAAACGAAAGGGAAATTTTCTAATCTGCTTTATCCATGTACTTGCATTTC
  AGACATGGACATGCTATTGTTATTTGGCTCATAACTGTTTCCAAATGTTAGTTATTATGGACCCAATTTA
  TTAACAACATTAGCTGATTTTTACCTATCAGTATTATTTTATTTCTTTTAGTTTATAGATCTGTGCAACA
  TTTTTGTACTGTATGTCTTCAAACCTGGCAGTATTAATACCCTTCTTACTGACATATGTACTTTTAGTTT
  TAGAAAACTTTTATATTTATGTGTCTTATTTTTATATTTCTTTATTTATTACACAGTGTAGTGTATAATA
  CTGTAGTTTGTATTAATACAATAATATATTTTAGTATGAAAATTTGGAAAGTTGATAAGATTTAAAGTAG
  AGATGCAATTGGTTCTCCTGCATTGAGATTTGATTTAACAGTGTTATGTTAACATTTATACTTGCCTTGG
  ACTGTAGAACAGAACTTAAATGGGAATGTATTAGTTTTACAACTACAATCAAGTCATTTTACCTTTACCC
  AGTTTTTAATATAAAACTTAAATTTTGAAATTCACTGTGTGACTAATAGCATGATGCTCTGCAGTTTTAT
  TAAGAAATCAGCCTAACCATACAACTCTCATTTCCTTAGTAAGCCAAATTAGGATTAACTTCTATAAACA
  GTGTTGGGAACAATGTTTAACATTTTGTGCCAATTTGTTCCTGTATTCATGTATGTAAGTTACAGATCTG
  ACTCTTCATTTTTAAGTTCCTTGTTACATCATGGTCATTTTCTAGTTTTTTACCAGACTCCCATCTCACA
  ATAAAATGCATCAACAAGCCTGAACTGCTGTCATTCTTTTCATCATTATCAGTATTTTCTTTGGAAAACT
  GTGAAATGGGGTACATTGTCATCCTGCATTTGATTCATCTTGAGCTGAATTTGGGTAACACTAAATGTTT
  TAGACATTCTCCACTAAATTATGGATTTTCTTGTGGCTAAATGTTTCTGGAGAGGTCAGAGTTGACAAAA
  CCTCTTCACAGGTTGCTCCTTCTTCCTGAAATCCTTAATCCTCCGCATTTCATGCTTCAGGTCATTTCAG
  GGAAGCCTGGGTTTAGATGCCTTTCTGACTCTCAGCTCCTGCACTTCTGTCATCATACCTCTGATACTAT
  TATTTATATTCCTTCCCCACTAGGAACAGGAACCACATTTGTCATAGTCACTCTCACATTCCTCACTGCC
  TAACAGGGTGCCTGGCATAAGTTGGGACAACAGATATTTGTTGAATAAAAATATAATTTGCATGTTTATG
  GAGCTCAGCTATGTTCTCACTTTTTTTGCTTCTAATTCCAGAATATATGTTAAATGATCTAATAATTTGA
  TTATTTTCTTATAAGTCTTATTAAACACTAGTCATAATAGACACAATAAATTATGCCTTCTTTTTCTATT
  GCC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001128128.2
• Summary: This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]
• Locus ID: 6935