Apolipoprotein L 1 (APOL1) (NM_003661) Human 3' UTR Clone

CAT#: SC215050

3' UTR clone of apolipoprotein L 1 (APOL1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: APOL1
• Synonyms: APO-L; APOL; APOL-I; FSGS4
• ACCN: NM_003661
• Insert Size: 1522 bp
• Sequence Data:
  Insert Sequence

  >SC215050 3’UTR clone of NM_003661
  The sequence shown below is from the reference sequence of NM_003661. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAGATTCTGCAGGCGGACCAAGAACTGTGACCACAGGGCAGGGCAGCCACCAGGAGAGATATGCCTGGC
  AGGGGCCAGGACAAAATGCAAACTTTTTTTTTTTTCTGAGACAGAGTCTTGCTCTGTCGCCAAGTTGGA
  GTGCAATGGTGCGATCTCAGCTCACTGCAAGCTCTGCCTCCCGTGTTCAAGCGATTCTCCTGCCTTGGC
  CTCCCAAGTAGCTGGGACTACAGGCGCCTACCACCATGCCCAGCTAATTTTTGTATTTTTAATAGAGAT
  GGGGTTTCACCATGTTGGCCAGGATGGTCTCGATCTCCTGACCTCTTGATCTGCCCACCTTGGCCTCCC
  AAAGTGCTGGGATTACAGGCGTGAGCCATCGCTTTTGACCCAAATGCAAACATTTTATTAGGGGGATAA
  AGAGGGTGAGGTAAAGTTTATGGAACTGAGTGTTAGGGACTTTGGCATTTCCATAGCTGAGCACAGCAG
  GGGAGGGGTTAATGCAGATGGCAGTGCAGCAAGGAGAAGGCAGGAACATTGGAGCCTGCAATAAGGGAA
  AAATGGGAACTGGAGAGTGTGGGGAATGGGAAGAAGCAGTTTACTTTAGACTAAAGAATATATTGGGGG
  GCCGGGTGTAGTGGCTCATGCCTGTAATCCGAGCACTTTGGGAGGCCAAGGCGGGCGGATCACGAGGTC
  AGGAGATCGAGACCATCCTGGCTAACACAGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGCCG
  GGCATGGTGGCGGGCGCCTGTAGTTCCAGCTAACTGGGCGGCTGAGGCAGGAGAATGGCGTGAACCTGG
  GAGGTGGAGCTTGCAGTGAGCCGAGATATCGCCACTGCACTCCAGCCTGGGTGACAGAGCGAGACTCCA
  TCTCAAAAAAAAAAAAAAAAAGAATATATTGACGGAAGAATAGAGAGGAGGCTTGAAGGAACCAGCAAT
  GAGAAGGCCAGGAAAAGAAAGAGCTGAAAATGGAGAAAGCCCAAGAGTTAGAACAGTTGGATACAGGAG
  AAGAAACAGCGGCTCCACTACAGACCCAGCCCCAGGTTCAATGTCCTCCGAAGAATGAAGTCTTTCCCT
  GGTGATGGTCCCCTGCCCTGTCTTTCCAGCATCCACTCTCCCTTGTCCTCCTGGGGGCATATCTCAGTC
  AGGCAGCGGCTTCCTGATGATGGTCATTGGGGTGGTTGTCATGTGATGGGTCCCCTCCAGGTTACTAAA
  GGGTGCATGTCCCCTGCTTGAACACTGAAGGGCAGGTGGTGGGCCATGGCCATGGTCCCCAGCTGAGGA
  GCAGGTGTCCCTGAGAACCCAAACTTCCCAGAGAGTATGTGAGAACCAACCAATGAAAACAGTCCCATC
  GCTCTTACCCGGTAAGTAAACAGTCAGAAAATTAGCATGAAAGCAGTTTAGCATTGGGAGGAAGCTCAG
  ATCTCTAGAGCTGTCTTGTCGCCGCCCAGGATTGACCTGTGTGTAAGTCCCAATAAACTCACCTACTCA
  TCAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003661.4
• Summary: This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]
• Locus ID: 8542
• MW: 57