PRMT4 (CARM1) (NM_199141) Human 3' UTR Clone

CAT#: SC211820

3' UTR clone of coactivator-associated arginine methyltransferase 1 (CARM1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CARM1
• Synonyms: PRMT4
• ACCN: NM_199141
• Insert Size: 1348 bp
• Sequence Data:
  Insert Sequence

  >SC211820 3’UTR clone of NM_199141
  The sequence shown below is from the reference sequence of NM_199141. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCGACCAACACCATGCACTACGGGAGCTAGGGGCCCGCCCCGCGGACTGACAGCACCAGGAAACCAAAT
  GATGTCCCTGCCCGCCGCCCCCGCCGGGCGGCTTTCCCCCTTGTACTGGAGAAGCTCGAACACCCGGTC
  ACAGCTCTCTTTGCTATGGGAACTGGGACACTTTTTTACACGATGTTGCCGCCGTCCCCACCCTAACCC
  CCACCTCCCGGCCCTGAGCGTGTGTCGCTGCCATATTTTACACAAAATCATGTTGTGGGAGCCCTCGTC
  CCCCCTCCTGCCCGCTCTACCCTGACCTGGGCTTGTCATCTGCTGGAACAGGCGCCATGGGGCCTGCCA
  GCCCTGCCTGCCAGGTCCCTTAGCACCTGTCCCCCTGCCTGTCTCCAGTGGGAAGGTAGCCTGGCCAGG
  CGGGGCCTCCCCTTCGACGACCAGGCCTCGGTCACAACGGACGTGACATGCTGCTTTTTTTAATTTTAT
  TTTTTTATGAAAAGAACCAGTGTCAATCCGCAGACCCTCTGTGAAGCCAGGCCGGCCGGGCCGAGCCAG
  CAGCCCCTCTCCCTAGACTCAGAGGCGCCGCGGGGAGGGGTGGCCCCGCCGAGGCTTCAGGGGCCCCCT
  CCCCACCAAAGGGTTCACCTCACACTTGAATGTACAACCCACCCCACTGTCGGGAAGGCCTCCGTCCTC
  GGCCCCTGCCTCTTGCTGCTGTCCTGTCCCCGAGCCCCTGCAGGTCCCCCCCCGCCCCCCCACTCAAGA
  GTTAGAGCAGGTGGCTGCAGGCCTTGGGCCCGGAGGGAAGGCCACTGCCGGCCACTTGGGGCAGACACA
  GACACCTCAAGGATCTGTCACGGAAGGCGTCCTTTTTCCTTGTAGCTAACGTTAGGCCTGAGTAGCTCC
  CCTCCATCCTTGTAGACGCTCCAGTCCCTACTACTGTGACGGCATTTCCATCCCTCCCCTGCCCGGGAA
  GGGACCTTGCAGGGACCTCTCCCTCCAAAAAAAGAAAAAAAGAAAAAGAAAGAAAAAATAAATGAGGAA
  ACGTGTTGCAGCACAGGCAGTTTTCTTCTCCTTCTGCTCCCCTGTTTCTCATACCCCCAAACTCAGATG
  CTGGAGCTCAGGCCCGCCGTGTGTGCACCCAGGCAGGAGCGGGCGCTGTCCAGGCTGGGCCGCCCCCTT
  GGCTCTCCCTCCTGTTCCAGGGGAGCCATAGGAGGGAAAGCAGGTGGCCCGGGGGGGATATGGGGGCCC
  CAGCCCTGTCCCAAAGCTCCCTGCTCGGCTGCCCCTCGCCCGCCTTTATATAAATTCTCTGAATCACCT
  TTGCATAGAAAATAAAAGTGTTTGCTTTGTAAGAAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_199141.2
• Summary: This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9. [provided by RefSeq, Aug 2013]
• Locus ID: 10498
• MW: 48.6