AGO3 (NM_177422) Human 3' UTR Clone

CAT#: SC209419

3' UTR clone of eukaryotic translation initiation factor 2C 3 (EIF2C3) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: AGO3
• Synonyms: EIF2C3
• ACCN: NM_177422
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC209419 3’UTR clone of NM_177422
  The sequence shown below is from the reference sequence of NM_177422. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GATACCTTACGCACAATGTACTTCGCTTAAATAGTCCAAGTATATTCTCTGAGAGGAAGTACTGAAAGA
  TGAATTGACATACAACGTATGTTTCCAGTGAAGTCAATTGAGTAAGGACACCTCCAGCCATACAGAAAC
  CAACACTGTGTGGGGGCCAAGGTCTGATCCTTATGTTAATACAAGGAAGATTGTTTACTTCATCAAGGA
  ACACAGCATCATTATGCAATATGAAACCAGCCAACTGCTTTTTGTGCGGTCTCCTATAGGAAGTATCGC
  AATTGTTTTGTTTTCATTTCTTGTAGTCTAACCCTTTTAATGCCTTTACCTCAAGTTGCTTGGCAGCAC
  AACTATCTTTGCAAAAAAAAGTAAAGAAAAAGTAAATGATGGTTTAAAAAATACACACCTTCATGAATA
  ATCAAAGTGATTTTTCAGAATTATGTGTGCAAAAAATTAATGTGCATTCATATATTCTTGTAAAAGGTG
  TCTGTGTATTTTTAAAATATATACATCCATACTTCATATGCATATATATCTAGATCTGGATTGATAATA
  GATATATATGTGTCTGTTATATATTTTAGAGTTCATTCCATTGGGGAATTTTCTTTCCCTTTTATTCTA
  CCCCCACTACCGCCTTTATTTCTCTATTTCCCTTGCCTTCATCACCTACATTTTTTTCCCAGTCCTACC
  AGTGACATTCAAATGTTGATGTATCTGGTTCGTTTGAATATAAAATATGGCAAACTAGAATTCTACTTT
  TATTTTGCATACCCTACTCCTGACTCCTATCTCATACACAATTCTGAGTTCTCAACTCTTGATATTGCT
  GTCTTAAGAAACAAAATTTTATATATATATGTATACATACATACACAGACACAGACACACACATATATA
  TACATAAAATAACTTTCTGTGAGAGTGCGTCTCTCCCCTCCCACTCAGAACATACATTTACCATTTGCG
  CAATTTGTATATAAGCAGTAGCAACCTGAACTATTCTTTTTCTTTTTTCTTTTTCTTTTCTTTCTTTTT
  TTTTTTTAATGAGACAGAGCCTCGCTCTGTCACCAGGCTGGAGTGCAGTGGCGAGATCTCGGCTCACTG
  CAACTTCCGCCTCTCGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGTTTACAGGCAT
  GTGCCACCACACCCAGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCGTGTTGGCCAGGATGG
  TCTCGATCTCTTGACCTTGTGATCCACCCGCCTCGACCTCCCAAAGTGCTGGGATTACAGGCATGAGCC
  ACCGCACCCGGCCTACTATTCTTTTTCTTTAGTGAGTTTGTTAAAGCTGCTATAAATATAAAACCTGTC
  AGAAGTTGTAACTTTCTCTCTGGTAAGTGATGTGTATTCTGAAGCCTTCTGTTTCTATGGGTTATACAT
  GGAAATTCTTTAAAGGTTTTATAAAGGGTAAATATTTTAAAAGATTAGAGCTCTGTAGGGTTGAGACCT
  CTGAAATTGAGTAGAGAGAAGAGTTTGTTGAGGGATTTTTTTGTTTTGTTTTTTGATAGTTTACTTGCT
  GCCTCTCATACCTTAATGTGATTTTCATATATTTTTTATATATATATATGTATGTGTGTGTATATAAAC
  ACATATGTATGTGTTTGGAAGTATAGCTTCCTTTTCTTTTATATTTATTAGAATAGTGCTTTAATAATT
  ATAGAATTGTATTGGCAGCATCTTTTATAGAAATATAATCATGTTTATTTTAAGAACTGACAACTTGTT
  TTTGCTATCTTTTAGTGCAATAAGCAGTTTTAAAGGAAAGTTGTGTCTGCATCTTTACCATGGATAGTA
  GGAGGAACAGGCACCTCCATAGTTTAAAAGGGCAAAGAATACAGCCTTAATTTCAGAAGGAAAGTTTTG
  TAATTTTCAGTTTTAAAGCATATCTTTTAAAATTCATCATGATAAAAGGCTGGACGCGGTGGCTCATG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_177422.3
• Summary: This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 192669
• MW: 78.2