Carrier-free (BSA/glycerol-free) DDK (FLAG) mouse monoclonal antibody, clone OTI11C3

CAT#: CF180144

Carrier-free (BSA/glycerol-free) DDK (FLAG) mouse monoclonal antibody, clone OTI11C3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY YPEL3 (RC208686; Lane 1), CYSLTR1 (RC207763; Lane 2), SEMA3D (RC216032; Lane 3), Dmp1 (RC222208; Lane 4) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDK (1:4000).

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  Immunofluorescent staining of 293T cells transfected by human CTAG1B plasmid(RC213318) using anti-DDK antibody (TA180144/green, right) compared to an Isotype control(Left).(1:400)

Specifications

Product Data

• Clone Name: OTI11C3
• Applications: ICC, IF, IHC, WB
• Recommended Dilution: WB 1:4000
• Target: DDK (FLAG tag): DYKDDDDK
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide coupled to KLH
• Isotype: IgG1
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: Stable for 1 year at -20°C from date of shipment.
• Note: This anti-DDK (FLAG) antibody recognizes both N-terminus and C-terminus DDK tags. It is recommended for Western blot only.