Rabbit IgG (H+L chain) Goat Polyclonal Antibody

CAT#: R1364B

Rabbit IgG (H+L chain) goat polyclonal antibody, Biotin, Lyophilized

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western Blot: 1/5,000-1/20,000 for Western blots.
  ELISA: 1/50,000-1/200,000.
  Immunohistochemistry: 1/1,000-1/5,000.
  
  
• Reactivities: Rabbit
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Rabbit IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide
  Label: Biotin
  State: Lyophilized
  State: Lyophilized purified Ig fraction
  Label: Conjugation chemistry: N-hydroxysuccinimide (NHS)ester. Label MW: 455
• Form: Lyophilized
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: Biotin
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.