MSK1 (RPS6KA5) Rabbit Polyclonal Antibody

CAT#: TA392652

MSK1 (phospho-S360) polyclonal antibody

Product Images

Western blot (WB) analysis of p-MSK1 (S360) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate Lane2:MCF-7 treated with LPS(100ng/ml) for 5 minutes whole cell lysate Lane3:MCF-7 treated with LPS(100ng/ml) for 30 minutes whole cell lysate Lane4:AML-12 whole cell lysate(40ug) Lane5:H9C2 whole cell lysate(40ug)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of Serine 360.
• Specificity: MSK1 (phospho-S360) polyclonal antibody detects endogenous levels of MSK1 only when phosphorylated at Ser360.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 90 kDa
• Gene Name: ribosomal protein S6 kinase A5
• Database Link:
  Entrez Gene 9252 Human
  UniProt ID O75582
• Background: MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress. Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain.
• Synonyms: 90 kDa ribosomal protein S6 kinase 5; MSK1; Nuclear mitogen- and stress-activated protein kinase 1; Ribosomal protein S6 kinase alpha-5; RPS6KA5; RSK-like protein kinase; RSKL; S6K-alpha-5
• Note: For research use only, not for use in diagnostic procedure.