NR1D1 Rabbit Polyclonal Antibody

CAT#: TA392606

Rev-Erbα polyclonal antibody

Product Images

Western blot (WB) analysis of Rev-Erbα polyclonal antibody at 1:10000 dilution Lane1:U-87MG whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:The Lung tissue lysate of Pig(40ug) Lane4:HepG2 whole cell lysate(40ug)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:5000~1:10000
• Reactivities: Human, Pig
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to Human Rev-Erbα.
• Specificity: Rev-Erbα polyclonal antibody detects endogenous levels of Rev-Erbα protein.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 78 kDa
• Gene Name: nuclear receptor subfamily 1 group D member 1
• Database Link:
  Entrez Gene 9572 Human
  UniProt ID P20393
• Background: Reverse orientation c-erbA gene α (Rev-erbα, EAR-1, or NR1D1) is a widely expressed member of the orphan nuclear receptor family of proteins. Rev-erbα is highly expressed in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation. Expression increases during differentiation in adipocytes and ectopic expression of Rev-erbα potentiates the adipocyte differentiation of 3T3-L1 cells. In addition, expression oscillates with circadian rhythm in liver cells and Rev-erbα regulates expression of BMAL1, ApoA-I and ApoC-III, all key regulators of circadian rhythm. Phosphorylation of Rev-erbα Ser55 and Ser59 by GSK-3β appears to stabilize Rev-erbα protein levels and is important for synchronizing and maintaining the circadian clock. Rev-erbα also regulates inflammation by targeting the NF-κB responsive genes IL-6 and COX-2. Rev-erbα lacks the activation function 2 domain required for ligand-dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co-repressor (N-CoR)/HDAC3 complex to target genes to repress transcription.
• Synonyms: EAR-1; Nuclear receptor subfamily 1 group D member 1; Rev-erbA-alpha; V-erbA-related protein 1
• Note: For research use only, not for use in diagnostic procedure.