CD35 (CR1) Rabbit Monoclonal Antibody [Clone ID: HB-8592]

CAT#: TA386153

Recombinant Anti-CD35 (Clone HB-8592)

Product Images

Flow-cytometry using the anti-CD35 antibody HB-8592 (TA386153). Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (clone 4-4-20; isotype control, black line) or the rabbit IgG1 version of HB-8592 (TA386153, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: HB-8592
• Applications: FC
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Animals were immunized with purified CR1 from human erythrocytes. Spleen cells were fused with P3X63Ag8.653 myeloma cells.
• Specificity: The antibody binds to a site distinct from the C3b binding site, and can be used to measure the number of receptors despite occupancy by ligand.
  
  This antibody has been used for analysis of the expression of the CR1 receptor on human leukemia-derived CD4+ T cell lines by flow cytometry (Yaskanin 1995).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: complement component 3b/4b receptor 1 (Knops blood group)
• Database Link:
  Entrez Gene 1378 Human
  UniProt ID P17927
• Synonyms: C3BR; C4BR; CD35; KN
• Note: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.