CDK4 Rabbit Monoclonal Antibody [Clone ID: R04-4I5]

CAT#: TA383968

CDK4 Rabbit monoclonal Antibody

Product Images

Western blot analysis of Cdk4 in K562, C6, 3T3, Hela lysates using CDK4 antibody.

• 

  Immunohistochemistry of Cdk4 in paraffin-embedded Human lung cancer tissue using Cdk4 Rabbit mAb at dilution 1/50

Specifications

Product Data

• Clone Name: R04-4I5
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/50
  ICC/IF: 1/50
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Recombinant protein of human Cdk4
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 34 kDa; Observed MW: 34 kDa
• Gene Name: cyclin-dependent kinase 4
• Database Link:
  Entrez Gene 1019 Human
  UniProt ID P11802
• Background: Swiss-Prot Acc.P11802.Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
• Synonyms: CMM3; MGC14458; PSK-J3