EGLN2 Rabbit Polyclonal Antibody

CAT#: TA375766

Prolyl hydroxylase PHD1 (EGLN2) Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Prolyl hydroxylase PHD1 (Prolyl hydroxylase PHD1 (EGLN2)) antibody (TA375766) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug Prolyl hydroxylase PHD1 (Prolyl hydroxylase PHD1 (EGLN2)) antibody (TA375766). Western blot was performed from the immunoprecipitate using Prolyl hydroxylase PHD1 (Prolyl hydroxylase PHD1 (EGLN2)) antibody (TA375766) at a dilition of 1:1000.

Specifications

Product Data

• Applications: IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IP,1:20 - 1:50
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human Prolyl hydroxylase PHD1 (Prolyl hydroxylase PHD1 (EGLN2)) (NP_444274.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 40kDa/43kDa
• Gene Name: egl-9 family hypoxia inducible factor 2
• Database Link:
  Entrez Gene 112398 Human
  UniProt ID Q96KS0
• Background: The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.
• Synonyms: DKFZp434E026; EIT6; HIF-PH1; HIFPH1; HPH-3; PHD1