B7-2 (CD86) Rabbit Polyclonal Antibody

CAT#: TA374490

CD86 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using CD86 antibody (TA374490) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 90s.

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  Immunofluorescence analysis of THP-1 cells using CD86 Polyclonal Antibody (TA374490) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of rat spleen using CD86 Polyclonal Antibody (TA374490) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of mouse spleen using CD86 Polyclonal Antibody (TA374490) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug CD86 antibody (TA374490). Western blot was performed from the immunoprecipitate using CD86 antibody (TA374490) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human CD86.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 12kDa/24kDa/28kDa/31kDa/37kDa
• Gene Name: CD86 molecule
• Database Link:
  Entrez Gene 942 Human
  UniProt ID P42081
• Background: This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
• Synonyms: B7-2; B70; BU63; CD28LG2; FUN-1; LAB72; MGC34413