ACSL1 Rabbit Polyclonal Antibody

CAT#: TA373211

ACSL1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ACSL1 antibody (TA373211) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

• 

  Immunofluorescence analysis of C6 cells using ACSL1 Rabbit pAb (TA373211) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of NIH-3T3 cells using ACSL1 Rabbit pAb (TA373211) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• 

  Immunoprecipitation analysis of 300ug extracts of Raji cells using 3ug ACSL1 antibody (TA373211). Western blot was performed from the immunoprecipitate using ACSL1 antibody (TA373211) at a dilition of 1:1000.

Specifications

Product Data

• Applications: ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 46-275 of human ACSL1 (NP_001273637.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 76kDa/77kDa
• Gene Name: acyl-CoA synthetase long-chain family member 1
• Database Link:
  Entrez Gene 2180 Human
  UniProt ID P33121
• Background: The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene.
• Synonyms: ACS1; FACL1; FACL2; LACS; LACS1; LACS2