B7-1 (CD80) Mouse Monoclonal Antibody [Clone ID: 12D9]

CAT#: TA355082

Mouse Monoclonal Anti-CD80 Antibody [12D9]

Product Images

Western blot analysis of CD80 in overexpressing HEK293 cells CD80 antibody at 0.25 and 0.5ug/ml

• 

  Immunohistochemistry of CD80 in human stomach carcinoma tissue with CD80 antibody at 5ug/ml.

• 

  Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 5ug/ml.

• 

  Immunofluorescence of CD80 in transfected HEK293 cells with CD80 antibody at 2ug/ml.

• 

  Immunofluorescence of CD80 in human stomach carcinoma tissue with CD80 antibody at 20ug/ml.

• 

  Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 2ug/ml.

• Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1ug/ml. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1ug/ml.

• Flow cytometry analysis of CD80 overexpressing HEK293 cells using CD80 antibody and control mouse IgG antibody at 10ug/ml. Blue: Untransfected HEK293 cells. Yellow: CD80 overexpressing HEK293 cells.

Show all 8 images

Specifications

Product Data

• Clone Name: 12D9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 0.5-1µg/mL.IHC starting at 2-5µg/mL.IF start at 5µg/mL.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: CD80 antibody was raised against the extracellular domain of human CD80.
• Formulation: CD80 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: CD80 Antibody is supplied as protein A purified IgG1.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 32 kDa; Observed: 50 kDa
• Gene Name: CD80 molecule
• Database Link:
  NP_005182
  Entrez Gene 941 Human
  UniProt ID P33681
• Background: CD80 Antibody: CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
• Synonyms: B7; BB1; CD28LG; CD28LG1; LAB7
• Note: CD80 antibody can be used for detection of CD80 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 µg/mL. For immunofluorescence start at 5µg/mL.