DBF4 Rabbit Polyclonal Antibody

CAT#: TA354621

Rabbit anti DBF4 Polyclonal Antibody

Product Images

WB: The tissue extract derived from mouse spleen was immunoblotted by Rabbit anti-DBF4 at 1:500. An immunoreactive band is observed ~77 kDa.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Rat, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to the N-terminus of DBF4 protein. This sequence is identical to human, rat and mouse.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~77 kDa
• Gene Name: DBF4 zinc finger
• Database Link:
  NP_006707
  Entrez Gene 27214 MouseEntrez Gene 312046 RatEntrez Gene 10926 Human
  UniProt ID Q9UBU7
• Background: The human DBF4/ASK protein contains a putative site for phosphorylation by CDK, PEST protease cleavage sites, nuclear localization signals and a short-looped zinc finger-like domain. DBF4 interacts physically with CDC7 and MCM2 as a cyclin-like regulatory subunit of mammalian CDC7, thus it plays a key role in the regulation of DNA replication during the cell cycle in all eukaryotes. The DBF4/ASK is also identified as a novel determinant for melanoma development. The up-regulation of ASK/DBF4 is a novel molecular determinant with prognostic relevance that confers a proliferative advantage in melanoma.
• Synonyms: ASK; CHIF; DBF4A; ZDBF1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle