beta III Tubulin (TUBB3) Mouse Monoclonal Antibody [Clone ID: 2E9]

CAT#: TA336754

Mouse Monoclonal Tubulin Beta 3 Antibody (2E9)

Product Images

Simple Western: beta-III Tubulin Antibody (2E9) TA336754 - Lane view shows a specific band for Tubulin Beta 3 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (2E9) TA336754 - Analysis of PANC-1 cells using Tubulin Beta 3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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  Flow Cytometry: beta-III Tubulin Antibody (2E9) TA336754 - Analysis of A549 cells using anti-Tubulin Beta 3 mAb (green) and negative control (purple).

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  Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (2E9) TA336754 - Confocal analysis of SHSY5Y cells using Tubulin Beta 3 antibody (TA336754, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

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  Western Blot: beta-III Tubulin Antibody (2E9) TA336754 - Analysis of Tubulin Beta 3 mouse mAb against (1) HepG2, (2) A549, and (3) Hela cell lysates.

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Specifications

Product Data

• Clone Name: 2E9
• Applications: ELISA, FC, ICC/IF, IHC, Simple Western, WB
• Recommended Dilution: Simple Western: 1:500, Flow Cytometry: 1:200-1:400, ELISA: 1:10000, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Western Blot: 1:500-1:2000, Immunohistochemistry
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human Tubulin Beta 3 expressed in E. coli. [UniProt# Q13509]
• Formulation: Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ascites
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50 kDa
• Gene Name: tubulin beta 3 class III
• Database Link:
  NP_006077
  Entrez Gene 246118 RatEntrez Gene 10381 Human
  UniProt ID Q13509
• Background: Tubulin Beta 3 is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of Tubulin Beta 3 is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. In adult tissues, the distribution of Tubulin Beta 3 is almost exclusively neuron-specific.
• Synonyms: beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; TUBB4
• Note: This Tubulin Beta 3 (2E9) antibody is useful in Western Blot, Immunocytochemistry/Immunofluorescence, Flow cytometry and ELISA.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection