CHRNA3 Rabbit Polyclonal Antibody

CAT#: TA321194

Anti-CHRNA3 Rabbit Polyclonal Antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 40 μg Lane: Hela cells Primary antibody: TA321194 (CHRNA3 Antibody) at dilution 1/600 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA321194 (CHRNA3 Antibody) at dilution 1/10 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA321194 (CHRNA3 Antibody) at dilution 1/10, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1000-5000
  WB positive control: Hela cells
  IHC: 15-50
  Positive control: Human ovarian cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 69-83 amino acids of Human cholinergic receptor, nicotinic, alpha 3 (neuronal)
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55 kDa
• Gene Name: cholinergic receptor nicotinic alpha 3 subunit
• Database Link:
  NP_000734
  Entrez Gene 25101 RatEntrez Gene 110834 MouseEntrez Gene 1136 Human
  UniProt ID P32297
• Background: This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit; as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.
• Synonyms: LNCR2; NACHRA3; PAOD2

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane