Apolipoprotein E (APOE) Mouse Monoclonal Antibody

CAT#: TA309573

Mouse Monoclonal ApoE4 Antibody (4E4)

Product Images

Immunocytochemistry/Immunofluorescence: ApoE4 Antibody (4E4) TA309573 - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ApoE4 (4E4) at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Specifications

Product Data

• Applications: ELISA, ICC/IF, IP, WB
• Recommended Dilution: Western Blot: 1:1000-1:2000, Immunoprecipitation: 1:10-1:500, Immunocytochemistry/ Immunofluorescence: 2-5 ug/ml, ELISA: 1:100-1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Synthetic peptide made to an internal region of human ApoE4 (within residues 100-150).
• Formulation: Tris-glycine, 150mM NaCl pH 7.5 and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: apolipoprotein E
• Database Link:
  NP_000032
  Entrez Gene 348 Human
  UniProt ID P02649
• Synonyms: AD2; APO-E; LDLCQ5; LPG

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Secreted Protein, Stem cell - Pluripotency
• Protein Pathways: Alzheimer's disease