GRB2 Rabbit Polyclonal Antibody

CAT#: TA302011

Rabbit Polyclonal Antibody against GRB2 (Y209)

Product Images

Western blot analysis of lysate from Ramos cell line, using GRB2 Antibody (pY209) (Cat. #TA302011). TA302011 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

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  Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GRB2 Antibody (Y209), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:10~50
• Reactivities: Human (Predicted: Mouse, Rat, Chicken, Xenopus)
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This GRB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-216 amino acids from human GRB2.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25206 Da
• Gene Name: growth factor receptor bound protein 2
• Database Link:
  NP_002077
  Entrez Gene 14784 MouseEntrez Gene 81504 RatEntrez Gene 2885 Human
  UniProt ID P62993
• Background: GRB2 binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway.
• Synonyms: ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084; NCKAP2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Prostate cancer, Renal cell carcinoma, T cell receptor signaling pathway