CD14 Mouse Monoclonal Antibody [Clone ID: MEM-18]

CAT#: SM3009B

CD14 mouse monoclonal antibody, clone MEM-18, Biotin

Product Images

Fig. 1. Western Blotting analysis (non-reducing conditions) of over-expressed human CD14 using anti-CD14 (MEM-18). Lane 1: whole cell lysate HEK 293 transfected with empty vector; Lane 2: tissue culture supernatant collected after cultivation of HEK 293 transfected with human CD14 cDNA; Lane 3: whole cell lysate of HEK 293 transfected with human CD14 cDNA

• 

  Surface staining of human peripheral blood leukocytes using anti-human CD14 (clone MEM-18) biotin.

Specifications

Product Data

• Clone Name: MEM-18
• Applications: FC, WB
• Recommended Dilution: Suitable for Indirect Immunofluorescence analysis by Flow Cytometry: 1/1000.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria
• Specificity: The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.
• Formulation: PBS, pH~7.4
  Label: Biotin
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
  Label: Conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Concentration: lot specific
• Conjugation: Biotin
• Storage:

  Store undiluted at 2-8°C.
  DO NOT FREEZE!
  

• Stability: Shelf life: one year from despatch.

• Gene Name: CD14 molecule
• Database Link:
  Entrez Gene 929 Human
  UniProt ID P08571
• Background: CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
• Synonyms: CD14