ACTN2 Mouse Monoclonal Antibody [Clone ID: EA-53]

CAT#: DM227

ACTN2 mouse monoclonal antibody, clone EA-53, Supernatant

Specifications

Product Data

• Clone Name: EA-53
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on formalin-fixed paraffin embedded tissues: diluted of 1:25-1:50 in an ABC method (30 minutes at room temperature)
  Recommended Positive Control: Skeletal muscle
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified rabbit skeletal alpha-actinin.
• Specificity: This antibody is specific for alpha-skeletal muscle actinin and alpha-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non-sarcomeric muscle elements. This antibody shows a wide range of cross reactivity with various mammalian species.
  
• Formulation: State: Supernatant
  State: Diluted Ascites containing sodium azide as preservative
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: actinin alpha 2
• Database Link:
  Entrez Gene 88 Human
  UniProt ID P35609
• Background: It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non- sarcomeric muscle elements. This antibody shows wide range of cross reactivity with various mammalian species.
• Synonyms: Alpha actinin skeletal muscle isoform 2