gamma Catenin (JUP) Mouse Monoclonal Antibody [Clone ID: OTI4D5]

CAT#: CF812398

Carrier-free (BSA/glycerol-free) JUP mouse monoclonal antibody,clone OTI4D5

Product Images

Western blot analysis of extracts (35ug) from cell lines and/or tissue lysates by using anti-JUP monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-JUP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812398) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-JUP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812398) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-JUP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812398) (1:2000)

Specifications

Product Data

• Clone Name: OTI4D5
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 500-745 of human JUP (NP_002221) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 81.6 kDa
• Gene Name: junction plakoglobin
• Database Link:
  NP_002221
  Entrez Gene 16480 MouseEntrez Gene 81679 RatEntrez Gene 3728 Human
  UniProt ID P14923
• Background: This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described. [provided by RefSeq, Jul 2008]
• Synonyms: CTNNG; DP3; DPIII; PDGB; PG; PKGB

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Pathways in cancer