STK23 (SRPK3) Mouse Monoclonal Antibody [Clone ID: OTI6F5]

CAT#: CF809462

Carrier-free (BSA/glycerol-free) SRPK3 mouse monoclonal antibody, clone OTI6F5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SRPK3 (Cat# RC215496, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SRPK3 (Cat# TA809462)(1:2000). Positive lysates LY415334 (100ug) and LC415334 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6F5
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 245-330 of human SRPK3 (NP_055185) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61.8 kDa
• Gene Name: SRSF protein kinase 3
• Database Link:
  NP_055185
  Entrez Gene 56504 MouseEntrez Gene 293854 RatEntrez Gene 26576 Human
  UniProt ID Q9UPE1
• Background: This gene encodes a protein kinase similar to a protein kinase which is specific for the SR (serine/arginine-rich domain) family of splicing factors. A highly similar protein has been shown to play a role in muscle development in mice. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]
• Synonyms: MSSK-1; MSSK1; STK23

Reference Data

• Protein Families: Druggable Genome, Protein Kinase