IKBKE Mouse Monoclonal Antibody [Clone ID: OTI1E4]

CAT#: CF807631

Carrier-free (BSA/glycerol-free) IKBKE mouse monoclonal antibody, clone OTI1E4 (formerly 1E4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IKBKE (RC212481, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IKBKE (1:500). Positive lysates LY415533 (100ug) and LC415533 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-IKBKE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807631) (1:150)

Specifications

Product Data

• Clone Name: OTI1E4
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 474-716 of human IKBKE(NP_054721) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 80.3 kDa
• Gene Name: inhibitor of nuclear factor kappa B kinase subunit epsilon
• Database Link:
  NP_054721
  Entrez Gene 9641 Human
  UniProt ID Q14164
• Background: IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]). [supplied by OMIM, Oct 2009]
• Synonyms: IKK-E; IKK-i; IKKE; IKKI

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway