Serine racemase (SRR) Mouse Monoclonal Antibody [Clone ID: OTI9E8]

CAT#: CF500949

Carrier-free (BSA/glycerol-free) SRR mouse monoclonal antibody, clone OTI9E8 (formerly 9E8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SRR (RC210359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SRR (TA500949). Positive lysates LY402897 (100ug) and LC402897 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-SRR monoclonal antibody (1:2000).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-SRR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500949, Dilution 1:50)

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  Anti-SRR mouse monoclonal antibody (TA500949) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SRR (RC210359).

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  Immunofluorescent staining of HepG2 cells using anti-SRR mouse monoclonal antibody (TA500949).

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  HEK293T cells transfected with either pCMV6-ENTRY SRR (RC210359) (Red) or empty vector control plasmid (Blue) were immunostained with anti-SRR mouse monoclonal (TA500949), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI9E8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: IHC 1:200, WB: 1:200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SRR(NP_068766) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.6 kDa
• Gene Name: serine racemase
• Database Link:
  NP_068766
  Entrez Gene 27364 MouseEntrez Gene 303306 RatEntrez Gene 63826 Human
  UniProt ID Q9GZT4
• Background: Catalyzes the synthesis of D-serine from L-serine
• Synonyms: ILV1; ISO1

Reference Data

• Protein Pathways: Glycine, serine and threonine metabolism