PYK2 (PTK2B) pTyr579 Rabbit Polyclonal Antibody

CAT#: AP55794PU-N

PYK2 (PTK2B) pTyr579 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from 3T3 cells (Lane 2) and HepG2 cells (Lane 3), using PYK2 (Phospho-Tyr579) Antibody. The lane on the left is treated with antigen-specific peptide.

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  Immunohistochemical analysis of paraffin-embedded human brain tissue using PYK2 (Phospho-Tyr579) antibody (left)or the same antibody preincubated with blocking peptide (right).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1:500~1:1000.
  Immunohistochemistry on paraffin sections: 1:50~1:100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of tyrosine 579(E-D-Y(p)-Y-K) derived from Human PYK2 (KLH-conjugated)
• Specificity: The antibody detects endogenous levels of PYK2 only when phosphorylated at tyrosine 579.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 116 kDa
• Gene Name: protein tyrosine kinase 2 beta
• Database Link:
  Entrez Gene 2185 Human
  UniProt ID Q14289
• Background: Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.
• Synonyms: PYK2, RAFTK, Protein tyrosine kinase 2 beta, Focal adhesion kinase 2, CAK-beta, CADTK