DLL3 (C-term) Rabbit Polyclonal Antibody

CAT#: AP51274PU-N

DLL3 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of DLL3 (arrow) in A375 cell line lysates (35ug/lane) using DLL3 antibody.

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  Immunohistochemistry anaysis of human brain tissue (Formalin-fixed, Paraffin-embedded) using DLL3 antibody. (C-term), follwed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis with 293 cell using DLL3 antibody. (C-term), followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution:

  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
  Western blot: 1/2000.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 519-548 aa) of human DLL3.
• Specificity: This antibody recognizes DLL3 at C-term .
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: delta like canonical Notch ligand 3
• Database Link:
  Entrez Gene 10683 Human
  UniProt ID Q9NYJ7
• Background: Delta-like protein 3 is a ligand for the Notch signaling pathway. It inhibits primary neurogenesis. DLL3 plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. Defects in DLL3 are the cause of Spondylocostal dysostosis autosomal recessive type 1 (SCDO1). Mutations in DLL3 gene cause truncal shortening relative to their limbs, which leads to abdominal protrusion, abnormal spinal curvature and sometimes a plagiocephaly-torticollis sequence. It may be required to divert neurons along a specific differentiation pathway.
• Synonyms: Delta-like protein 3, Delta-3
• Note: Molecular Weight: 64618 Da

Reference Data

• Protein Families: Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transmembrane
• Protein Pathways: Notch signaling pathway