GATA4 Rabbit Polyclonal Antibody

CAT#: AP20302PU-N

GATA4 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of GATA4 antibody (Cat.-No.: AP20302PU-N) in extracts from HeLa cells.

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  Immunohistochemistry analyzes of GATA4 antibody (Cat.-No.: AP20302PU-N) in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of GATA4 protein.
  (region surrounding Pro101)
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 48 kDa
• Gene Name: GATA binding protein 4
• Database Link:
  Entrez Gene 14463 MouseEntrez Gene 54254 RatEntrez Gene 2626 Human
  UniProt ID P43694
• Background: GATA4 is a 46 kDa member of the GATA family of zinc finger containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs.
• Synonyms: GATA-4, GATA-binding factor 4

Reference Data

• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors