HLA-DRA (C-term) Rabbit Polyclonal Antibody

CAT#: AP17467PU-N

HLA-DRA (C-term) rabbit polyclonal antibody

Product Images

Western blot analysis of HLA-DRA Antibody (C-term) in HL60 cell line lysates (35 ug/lane). HLA-DRA (arrow) was detected using the purified Pab.

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  Flow cytometric analysis of HL-60 cells using HLA-DRA Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: Western blot: 1:1000.
  Peptide ELISA: 1:1,000.
  Flow cytometry: 1:10 - 1:50.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 149-177 amino acids from the C-terminal region of human HLA-DRA.
• Specificity: This antibody detects HLA-DRA at C-term.
• Formulation: PBS with 0.44% (W/V) sodium azide
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: major histocompatibility complex, class II, DR alpha
• Database Link:
  Entrez Gene 3122 Human
  UniProt ID P01903
• Background: HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages).
• Synonyms: HLA-DRA1, MHC class II antigen DRA
• Note: Molecular weight: 28607 Da

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Hematopoietic cell lineage, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis