ABCB5 (N-term) Rabbit Polyclonal Antibody

CAT#: AP13085PU-N

ABCB5 (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-ABCB5 Antibody (N-term) in K562 cell line lysates (35 ug/lane). ABCB5 (arrow) was detected using the purified Pab.

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  TOP: Flow Cytometry using ABCB5 Antibody (N-Term) on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit as a secondary antibody. Analysis was done on an FC500 flow cytometer. BOTTOM: Flow Cytometry using ABCB5 Antibody (N-Term) on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit as a secondary antibody. Analysis was done on an FC500 flow cytometer.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/50-1/100.
  Flow Cytometry.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ABCB5.
• Specificity: This antibody recognizes ABCB5 (N-term).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ATP binding cassette subfamily B member 5
• Database Link:
  Entrez Gene 340273 Human
  UniProt ID Q2M3G0
• Background: ABCB5 is a plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. ABCB5 is also responsible for the resistance to doxorubicin of a subset of malignant melanomas. ABCB5 has more recently been identified as a marker for human malignant-melanoma-initiating cells.
• Synonyms: ABCB5alpha; ABCB5beta; EST422562
• Note: Calculated Molecular Weight: 89831 Da