CBL pTyr700 Rabbit Polyclonal Antibody

CAT#: AP08042PU-N

CBL pTyr700 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 3 Western blot analysis of extracts from HT29 cells untreated or treated with UV using c-Cbl (pTyr700) antibody

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  Figure 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Cbl pTyr700 Antibody

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  Figure 1. Immunofluorescence staining of methanol-fixed HeLa cells using c-Cbl pTyr700 antibody (Red).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunofluorescence: 1/100 - 1/200.
  Immunohistochemistry on Paraffin-Embedded Sections: 1/50 - 1/100.
  Western Blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human c-Cbl around the phosphorylation site of Tyrosine 770 (T-E-YP-M-T).
• Specificity: This Antibody detects endogenous levels of c-Cbl only when phosphorylated at Tyrosine 770.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Cbl proto-oncogene
• Database Link:
  Entrez Gene 867 Human
  UniProt ID P22681
• Background: The proto-oncogene c-CBL was initially identified as the cellular homologue of v-CBL oncogene that induces pre-B cell lymphomas and myeloid leukemias in mice. In more recent studies CBL has been shown to be a negative regulator of tyrosine kinase signaling. The ubiquitin ligase activity of CBL leads to the degradation of tyrosine kinases, thus attenuating the signal of receptors. Targets of CBL include activated protein tyrosine kinases belonging to the Src and Syk/Zap-70 families. An additional mechanism to attenuate receptor signaling is thought to be achieved by CBL’s interaction with downstream targets of tyrosine kinases, such as PI-3K and Vav.
• Synonyms: RING finger protein 55, CBL2, c-CBL