MAPKAP Kinase 3 (MAPKAPK3) Rabbit Polyclonal Antibody

CAT#: AP06739PU-N

MAPKAP Kinase 3 (MAPKAPK3) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of 3pK antibody in extracts from COLO205 cells.

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  Immunohistochemistry (IHC) analyzes of3pK antibody in paraffin-embedded human skeletal muscle tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
  
  
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 289-341 of Human MAPKAPK-3.
• Specificity: This antibody detects endogenous levels of 3pK protein.
  (region surrounding Arg319)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 42 kDa
• Gene Name: mitogen-activated protein kinase-activated protein kinase 3
• Database Link:
  Entrez Gene 7867 Human
  UniProt ID Q16644
• Background: The MAPKAP kinases (for MAP kinase activated protein kinases) are a group of MAP kinase substrates which are themselves kinases. In response to activation, the MAP kinases phosphorylate downstream components on a consensus Pro-X-Ser/Thr-Pro motif. Several kinases that contain this motif have been identifed and serve as substrates for the ERK and p38 MAP kinases. These include the serine/threonine kinases Rsk-1 (also designated MAPKAP kinase-1), Rsk-2 and Rsk-3, which are phosphorylated by ERK 1 and ERK 2. Similarly, p38 phosphorylates and activates the serine/threonine kinases MAPKAPK-2 and MAPKAPK-3 (also designated 3pK). The serine/threonine kinases Mnk1 and Mnk2 are substrates for both ERK and p38 MAP kinases.
• Synonyms: MAPK-activated protein kinase 3, MAPKAPK-3, MAPKAPK3