MMP1 Rabbit Polyclonal Antibody

CAT#: AP06224PU-N

MMP1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of MMP-1 antibody at 1/500 dilution Lane 1:Hela whole cell lysate treated with LPS Lane 2:Raw264.7 whole cell lysate treated with LPS Lane 3:PC12 whole cell lysate treated with LPS

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 411-460 of Human MMP-1.
• Specificity: This antibody detects endogenous levels of MMP-1 protein.
  (region surrounding His440)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 54 kDa
• Gene Name: matrix metallopeptidase 1
• Database Link:
  Entrez Gene 4312 Human
  UniProt ID P03956
• Background: The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-9 (also designated 92 kDa type IV collagenase or gelatinase B) has been shown to degrade bone collagens in concert with MMP-1 (also designated interstitial collagenase, fibroblast collagenase or collagenase-1), and cysteine proteases and may play a role in bone osteoclastic resorption. MMP-1 is downregulated by p53, and abnormality of p53 expression may contribute to joint degradation in rheumatoid arthritis by regulating MMP-1 expression.
• Synonyms: Interstitial collagenase, Matrix metalloproteinase-1, Fibroblast collagenase, MMP1, CLG