Cyclin A2 (CCNA2) Rabbit Polyclonal Antibody

CAT#: AP06079PU-N

Cyclin A2 (CCNA2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Cyclin A antibody (Cat.-No.: AP06079PU-N) at 1/500 dilution Lane 1:HEK293T cell lysate Lane 2:NIH-3T3 cell lysate Lane 3:Rat brain tissue lysate

• 

  Immunohistochemical analysis in paraffin-embedded human lung carcinoma tissue using Cyclin A2 antibody (Cat.-No. AP06079PU-N).

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Cyclin A2 protein (region surrounding Trp250).
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen (> 95% pure by SDS-PAGE).
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~52 kDa
• Gene Name: cyclin A2
• Database Link:
  Entrez Gene 890 Human
  UniProt ID P20248
• Background: The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34.
• Synonyms: Cyclin-A2, CCNA2, CCN1, CCNA, Cyclin A