BAD Rabbit Polyclonal Antibody

CAT#: AP06025PU-N

BAD rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Bad antibody at 1/500 dilution Lane 1:LO2 cell lysate Lane 2:Raw264.7 cell lysate Lane 3:Rat brain tissue lysate

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  Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using BAD / Bcl-2-like 8 antibody.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 112-163 of Human Bad.
• Specificity: This antibody detects endogenous levels of BAD / Bcl-2-like 8 protein (region surrounding Arg149).
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~24 kDa
• Gene Name: BCL2 associated agonist of cell death
• Database Link:
  Entrez Gene 572 Human
  UniProt ID Q92934
• Background: The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiologic conditions. Bcl-2 and several related proteins function to inhibit apoptosis, while other members of the Bcl-2 family, such as Bax and Bak, enhance cell death under various conditions. For instance, Bcl-xL represses cell death, while its shorter form, Bcl-xS, promotes apoptosis. A protein designated Bad exhibits homology to Bcl-2, limited to the BH1 and BH2 domains. Bad functions to dimerize with Bcl-xL and with Bcl-2, but not with Bax, Bcl-xS, Mcl-1, A1 or itself. In mammalian cells, Bad binds with greater affinity to Bcl-xL than to Bcl-2, and reverses the death repressor activity of Bcl-xL but not Bcl-2. Dimerization of Bad with Bcl-xL results in displacement of Bax from Bcl-xL:Bax complexes, thereby causing restoration of Bax-mediated apoptosis.
• Synonyms: BAD, BBC6, BCL2L8, Bcl-2-like protein 8, Bcl2-L-8