CAMK2A pThr286 Rabbit Polyclonal Antibody

CAT#: AP02526PU-S

CAMK2A pThr286 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot analysis of extract from 293 cells untreated or treated with PMA using CaMKII (Phospho-Thr286) Antibody

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  Figure 2. Immunofluorescence staining of paraffin-embedded human hippocampal region tissue from the falling sickness disease using CaMKII (phospho-Thr286) antibody (#, green).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500~1/1000.
  Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunofluorescence: 1/100~1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human CaMKII around the phosphorylation site of Threonine 286 (Q-E-Tp-V-D).
• Specificity: This antibody detects endogenous levels of CaMKII only when phosphorylated at Threonine 286.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity-Chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: calcium/calmodulin dependent protein kinase II alpha
• Database Link:
  Entrez Gene 815 Human
  UniProt ID Q9UQM7
• Background: CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. CAMK2A assembles into heterooligomeric complexes with other CAMK2 subunits.
  CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter release.
• Synonyms: CAMK2A, CAMK2B, CAMKA, KIAA0968, CaM-kinase II alpha chain, CaM kinase II subunit alpha, CaMK-II subunit alpha, CaM-kinase II beta chain, CaM kinase II subunit beta, CaMK-II subunit beta