BAD pSer112/75 Rabbit Polyclonal Antibody

CAT#: AP02366PU-N

BAD pSer112/75 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (phospho-Ser112) antibody.

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  Figure 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (phospho-Ser112) antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Suitable for use in Western blot (1:500~1:1000) and Immunohistochemistry (1:50~1:100).
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human BAD around the phosphorylation site of serine 112 (H-S-SP-Y-P).
• Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  BAD (phospho-Ser112) antibody detects endogenous levels of BAD only when phosphorylated at serine 112.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: BCL2 associated agonist of cell death
• Database Link:
  Entrez Gene 572 Human
  UniProt ID Q92934
• Background: Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
• Synonyms: BAD, BBC6, BCL2L8, Bcl-2-like protein 8, Bcl2-L-8