BAD pSer112/75 Rabbit Polyclonal Antibody

CAT#: AP01734PU-N

BAD pSer112/75 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of BAD pSer112 antibody (Cat.-No.: AP01734PU-N) in extracts from 293 cells treaed with Forskolin.

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  Immunohistochemistry (IHC) analyzes of BAD pSer112 antibody (Cat.-No.: AP01734PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of BAD pSer112 protein.
• Formulation: Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~ 24 kDa
• Gene Name: BCL2 associated agonist of cell death
• Database Link:
  Entrez Gene 572 Human
  UniProt ID Q92934
• Background: Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
• Synonyms: BAD, BBC6, BCL2L8, Bcl-2-like protein 8, Bcl2-L-8