MAPT / TAU pSer356 Rabbit Polyclonal Antibody

CAT#: AP01706PU-N

MAPT / TAU pSer356 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Tau pSer356 antibody (Cat.-No.: AP01706PU-N) in extracts from 293 cells treated with serum 10% 15'.

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  Immunohistochemistry (IHC) analyzes of Tau pSer356 antibody (Cat.-No.: AP01706PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human isoforms Tau-4 around the phosphorylation site of Serine 356
• Specificity: This antibody detects endogenous levels of Tau only when phosphorylated at Ser673, isoforms Tau-4 phosphorylated at Ser356, and other isoforms phosphorylated at the corresponding Site.
• Formulation: Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: 50-80 kDa
• Gene Name: microtubule associated protein tau
• Database Link:
  Entrez Gene 4137 Human
  UniProt ID P10636
• Background: Tau is a neuronal microtubule associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer's disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilisation of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Hyperphosphorylated tau is also found in a range of other central nervous system disorders. Numerous serine/threonine kinases, including GSK3 beta, PKA, Cdk5, and casein kinase II can phosphorylate Tau.
• Synonyms: MAPTL, MTBT1, Microtubule-associated protein tau, PHF-tau, Neurofibrillary tangle protein, Paired helical filament-tau