BAD pSer155/118 Rabbit Polyclonal Antibody

CAT#: AP01535PU-N

BAD pSer155/118 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p-BAD (pSer155) antibody in extracts from 293 cells treated with Forskolin.

• 

  Immunohistochemistry (IHC) analyzes of p-BAD (pSer155) antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1:10000~1:20000.
  WB: 1:500~1:1000.
  IHC: 1:50~1:200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: p-BAD (pSer155) antibody detects endogenous levels of p-BAD protein.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig
• Concentration: 1,0 mg/ml
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: BCL2 associated agonist of cell death
• Database Link:
  Entrez Gene 572 Human
  UniProt ID Q92934
• Background: The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiologic conditions. Bcl-2 and several related proteins function to inhibit apoptosis while other members of the Bcl-2 family, such as Bax and Bak, enhance cell death under various conditions. For instance, Bcl-xL represses cell death, while its shorter form, Bcl-xS, promotes apoptosis. A protein designated Bad exhibits homology to Bcl-2 limited to the BH1 and BH2 domains. Bad functions to dimerize with Bcl-xL and with Bcl-2, but not with Bax, Bcl-xS, Mcl-1, A1 or itself. In mammalian cells, Bad binds with greater affinity to Bcl-xL than to Bcl-2 and reverses the death repressor activity of Bcl-xL but not Bcl-2. Dimerization of Bad with Bcl-xL results in displacement of Bax from Bcl-xL:Bax complexes, thereby causing restoration of Bax-mediated apoptosis
• Synonyms: BAD, BBC6, BCL2L8, Bcl-2-like protein 8, Bcl2-L-8