HLA-DRA Mouse Monoclonal Antibody [Clone ID: IPO-10]

CAT#: AM32359PU-T

HLA-DRA mouse monoclonal antibody, clone IPO-10, Purified

Product Images

Formalin-paraffin human tonsil stained with HLA-DR MAb (Clone LN-3).

Specifications

Product Data

• Clone Name: IPO-10
• Applications: FC, IF, IHC
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Positive Control: Ramos, Daudi or HuT78 cells, Tonsil or lymph node.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: Spleen cells of a patient with hairy cell leukemia (Daudi cells).
• Specificity: MAb IPO-10 defines the antigen, which appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD37 and cym. It is expressed on resting B cells and than reappears and persists in cytoplasm and on cell surface until cytoplasmic Ig appears. It is a useful antibody for diagnostics of neoplasms of B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50% of cells).
  The MAb does not label T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again. This MAb labels B cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70% of B cell CLL and some B-NHL were also positive. IPO-10 reacts with AMML cells and in a majority of Hodgkins disease cases a significant percentage of affected lymph node cells were detected.
  Cellular Localization: Cell surface.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~36 kDa
• Gene Name: major histocompatibility complex, class II, DR alpha
• Database Link:
  Entrez Gene 3122 Human
  UniProt ID P01903
• Background: HLA DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of an alpha chain (36 kDa) and a beta chain (27 kDa). It is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T helper cells. They therefore have a critical role in the initiation of the immune response.
• Synonyms: HLA-DRA1, MHC class II antigen DRA