MRP1 (ABCC1) Rat Monoclonal Antibody [Clone ID: MRPr1]

CAT#: AM32053SU-N

MRP1 (ABCC1) rat monoclonal antibody, clone MRPr1, Supernatant

Specifications

Product Data

• Clone Name: MRPr1
• Applications: IF, IHC, WB
• Recommended Dilution:

  Flow Cytometry: Use at least 1/20-1/50.
  Cell permeabilization required with 10% lyzing solution followed by primairy antibody and anti-Rat-FITC.
  Western blotting: Use at least 1/20-1/50. Use an anti-Rat HRP.
  Immunocytochemistry: Use at least 1/20-1/50 on Aceton fixed cell preparation.
  Immunohistochemstry on Acetone-Fixed Frozen Sections: at least 1/20-1/50.
  Immunohistochemstry on Formalin-Fixed Paraffin Embedded Tissues: 1/20.
  Pretreatment (0.01M Citrate, 10 min. at 100°C) may increase the performance of this antibody.

• Reactivities: Human
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Bacterial fusion protein of MRP, containing a segment of 168 amino acids (aa 192-360) in the amino-proximal half of the protein.
• Specificity: This Monoclonal antibody MRPr1 reacts with an epitope of MRP, a 180-195 kD transmembrane transporter protein overexpressed in various Human non-P-glycoprotein MDR tumor cell lines.
  It does not cross-react with the Human MDR1 and MDR3 gene products (See Flens et al. 1994).
• Formulation: State: Supernatant
  State: Serum-Free Tissue Culture Supernatant
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for 3 months or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ATP binding cassette subfamily C member 1
• Database Link:
  Entrez Gene 4363 Human
  UniProt ID P33527
• Background: MRP1 (also known as ABCC, GS-X, ABC29) functions as a multispecific organic anion transporter, with (oxidized) glutathione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. It also transports drugs and other hydrophobic compounds in presence of glutathione. MRP1 protein is found predominantly in the heart, lung, small intestine, brain, and skin.
• Synonyms: Multidrug resistance-associated protein 1, LTC4 transporter